Normal or genetically modified cell(s) having magnetic nanoparticle(s) bound (affixed) to their surfaces and methods of delivery to target tissues, e.g. For treatment of disease and/or injury.

The present invention relates to processes and systems for preparing nanoparticles, cellular or viral membranes and/or cellular or viral membrane coated nanoparticles using or comprising, inter alia, a multi-inlet vortexing reactor, tangential flow filtration (TFF) and/or a high shear fluid processor such as a microfluidizer (or a microfluidizer processor). The present invention also relates to the nanoparticles, cellular or viral membranes and/or cellular or viral membrane coated nanoparticles prepared by the present processes and systems, and the uses and/or applications of the nanoparticles, cellular or viral membranes and/or cellular or viral membrane coated nanoparticles.

Disclosed are methods and compositions for diagnosis, imaging, and treating one or more mammalian diseases, including, for example, treatment, prophylaxis, and/or amelioration of one or more symptoms of a human cancer. Disclosed are compositions and methods for the treatment and amelioration of one or more symptoms of a disease, and in exemplary embodiments, for use as an immunotherapeutic.

The present disclosure is directed, in some embodiments, to methods and compositions of comprising a cell having a non-internalizing receptor, and a nanoparticle surface-modified with a ligand that binds to the non-internalizing receptor.

A method for targeted delivery of cargo to a target locus in a subject and components of the system. Generally, the method includes administering to the subject a cell that has an artificial saccharide-derived target presented on the surface of the cell, allowing the cell to localize to a target locus in the subject, then administering to the subject an agent that specifically binds to the artificial target. In some embodiments, the agent can include a therapeutic compound. In some embodiments, the agent can include a detectable label. In some of these embodiments, that method can further include detecting the detectable label.

A method for enhancing extracellular vesicle production is described. A peptide that induces polymer formation is incubated with a cell culture which results in enhanced EV production. The peptide penetrates the cells and subsequently polymerizes upon exposure to enzymes (e.g. phosphatase) within the cell. The cells that contain the newly formed polymers have an increased production of EVs. These EVs can be harvested using centrifugation techniques.

Methods and compositions disclosed herein relate to cancer immunotherapy, in particular preparation and use of antigen-specific T lymphocytes for immune cell therapies. Methods and compositions disclosed herein relate to the production of antigen-presenting cells and their use cell therapy and vaccines and, in particular, the preparation and use of antigen-specific T lymphocytes for cancer immunotherapies.

A modified vertebrate cell comprising a vertebrate cell encased in reversibly interlinked metal-organic framework (MOF) nanoparticles, and methods of making and using the modified cell, are provided.

Cells incorporating a synthetic molecule construct of the structure F—S.sub.1—S.sub.2-L where: F—S.sub.1 is an aminoalkylglycoside where F is a mono-, di-, tri- or oligo-saccharide and S.sub.1 is 2-aminoethyl, 3-aminopropyl, 4-aminobutyl, or 5-aminopentyl; S.sub.2 is —CO(CH.sub.2).sub.2CO—, —CO(CH.sub.2).sub.3CO—, —CO(CH.sub.2).sub.4CO— or —CO(CH.sub.2).sub.5CO—; and L is phosphatidylethanolamine.

The disclosure presented herein provides artificial receptors and a system comprising recombinant cells decorated with various labels and/or synthetic agents, wherein said labels and/or synthetic agents can be reversibly modified or removed from the cells. Also disclosed herein are methods for decorating and/or modifying the cells and methods for using thereof.