The producing method includes causing a cell suspension to flow into a tubular flow path; injecting a gel precursor into the flow path to cover the circumference of the cell suspension with the gel precursor; injecting a gas into the flow path to form, in the flow path, an alternating flow obtained from the gas and the cell suspension covered with the gel precursor; and injecting a gelating agent into the flow path and causing the gelating agent to join the alternating flow to the gel precursor.

Disclosed is a microcapsule which is used in tissue regeneration, which may be specifically directed to the damaged tissues, and which forms an extracellular matrix-like structure at a certain point and thus allows cell proliferation, and to the production method of such microcapsule.

An implantable containment apparatus for receiving and retaining a plurality of cells for insertion into a patient, such as into a tissue bed, is disclosed. The device includes a chamber having structural spacers therein to maintain an average distance between the first interior surface and the second interior surface of the chamber and to define at least one reservoir space for the placement of cells within the chamber.

Disclosed herein are hydrogel compositions comprising a triblock copolymer having a formula A-B-A, wherein A is a polycaprolactone (PCL) block or a polyvalerolactone (PVL) block and B is a polyethylene glycol (PEG) block. Also disclosed are methods of making a hydrogel comprising providing a photoinitiator and a triblock copolymer having a formula A-B-A, wherein the triblock copolymer comprises one or more ethylenically unsaturated moieties; and photocrosslinking the triblock copolymer, thereby forming a hydrogel. Also disclosed are methods of printing a three-dimensional (3D) article comprising extruding a printing composition from a deposition nozzle moving relative to a substrate, the printing composition comprising a photoinitiator and any herein disclosed triblock copolymer, wherein the triblock copolymer comprises one or more ethylenically unsaturated moieties; depositing one or more layers comprising the printing composition on the substrate; and photocrosslinking the triblock copolymer to form the printed 3D article.

An oral dissolving film containing nano- or micro-encapsulated bioactive material and methods of forming the film. The film may be prepared by dispensing a mixture of a film-forming agent, a crosslinking agent, a solution of nano- or micro-encapsulated bioactive material, and a photoinitiator into a plurality of wells in a tray using a 3D printer. The dispensed material is exposed to radiation in order to crosslink the material and form a film.

Described herein is a method for encapsulating single cells using alternating current electrospray technology in tip streaming mode. The encapsulation efficiency is over 80% and natural (alginate, collagen) and synthetic (NorHA) hydrogels and various cell types can be used. The encapsulated cells can be implanted and are protected from the host's immune response. In addition, the coating allows better tissue growth in laboratory cell cultures with a conformal mechanical support that allows molecular and nutrient transport.

A composite shell particle including a composite shell layer is provided. The composite shell layer is a hollow shell, wherein the composite shell layer includes a porous biological layer and a metallic layer. The porous biological layer is composed of an organic substance including a cell wall or a cell membrane of a bacteria or algae. The metallic layer is crosslinked with the porous biological layer to form the composite shell layer. The metallic layer includes at least one metal selected from the group consisting of iron, molybdenum, tungsten, manganese, zirconium, cobalt, nickel, copper, zinc, and calcium, and/or includes at least one selected form the group consisting of metal chelates, metal oxides, metal sulfides, metal chlorides, metal selenides, metal acid salt compounds, and metal carbonate compounds. A method of manufacturing the composite shell particle, and a biological material including the composite shell particle and the applications thereof are also provided.

Described herein are apparata and methods for growing cells in a manner that mimics the native three-dimensional environment. Cell cultures grown in the apparatus can be screened for inhibition by specific chemotherapeutics or other drugs.

Thin film devices, e.g., multilayer thin film devices, that encapsulate cells for transplantation into a subject are provided. Also provided are methods of using and methods of preparing the subject devices. The thin film devices include a first porous polymer layer and a second porous polymer layer that define a lumen therebetween and encapsulate a population of cells within the lumen. The thin film devices can promote vascularization into the lumen of the device via the pores in the first polymer layer and/or second polymer layer; limit foreign body response to the device; limit ingress of cells, immunoglobulins, and cytokines into the lumen via the first and the second polymer layers; and release from the first polymer layer and/or the second polymer layer molecules secreted by the population of cells.

Described herein are oral delivery systems for use in delivering live mammalian cells to the intestinal tract of an individual.