The invention provides a substrate for producing cell aggregates provided with a spot(s) comprising a copolymer containing recurring units derived from monomers represented by the following formulae (I) and (II):

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wherein U.sup.a1, U.sup.a2, R.sup.a1, R.sup.a2 and R.sup.b are as defined herein, on a substrate having an ability to inhibit adhesion of cells, wherein a completion time of forming cell aggregates after seeding cells is within 20 hours.

A cell treatment agent containing alginate sulfate as an active ingredient, and a set reagent for activating suspended or dormant cells, which is a combination reagent of the cell treatment agent and an activator containing polyvalent cations are provided.

The invention relates to a bioreactor including: a light source (200); a light sensor (300) facing said light source; a vat (100) that is placed between the light source (200) and the light sensor (300), said vat being intended to receive a culture medium comprising a cellular culture of photosynthetic microorganisms; a controller (400) connected to the light sensor (300) in order to control the vat (100) to obtain a chosen cellular-culture concentration (xi) in the culture medium during a working period, said light source (200) being capable of emitting incident light (L) of an input light intensity (Iin) in the direction of the vat (100), and the light sensor (300) being capable of measuring an output light intensity (Iout) and of transmitting data relating to this intensity (Iout) to the controller for the control of the vat; and a system (500) for controlling the light source (200), this system being arranged to adjust, during a period shorter than or equal to said working period, the input light intensity (Iin) to a setpoint value allowing a cellular stress to be induced in certain at least of said photosynthetic microorganisms.

A method for coating a solid surface with a recombinant spider silk protein capable of forming polymeric, solid structures is provided. The method is comprising the following steps: exposing the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming a surface layer of the recombinant spider silk protein adsorbed on the solid surface without formation of covalent bonds between the recombinant spider silk protein and the solid surface; and further exposing the surface layer of the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming an assembled silk structure layer of the recombinant spider silk protein on the surface layer; wherein the method does not include drying-in of spider silk protein.

In order to induce aging while cutting the costs associated with adding cytokines at different aging stages, in a system for aging induction including a first culture chamber for perfusing, with a culture medium, a subject of aging-induction, the subject of aging-induction being derived from a living organism; a second culture chamber for perfusing, with a culture medium, a secretor that secretes a cytokine; and a control device for aging induction including a detection unit, a memory unit and a control unit, a protocol for aging induction defining a procedure of aging induction is stored, and in the control unit, an aging state of the subject of aging-induction is detected with a detection unit, and based on the protocol for aging induction, a flow rate at which the culture medium containing the cytokine secreted by the secretor is supplied to the subject of aging-induction is controlled according to the aging state of the subject of aging-induction.

The present disclosure provides for a cell stabilizing medium which comprises gelatin. The cell stabilizing medium help maintain cell viability, e.g., after thawing of a biological material post-cryopreservation.

The present disclosure provides, in part, a cell culture medium supplement comprising at least one plant protein homologue of a serum protein, a cell culture medium comprising a serum-free base medium and one or more plant based proteins, and methods of growing cells in vitro and of producing cultured meat using the cell culture medium.

The invention relates to a method of predicting production stability and/or production instability of a clonal cell line, the method comprising the steps of a) growing two or more clonal cell lines in separate cell cultures b) karyotyping the cells in each cell culture; and c) deriving a genomic instability value from the karyotyping of step (b). The invention also relates to methods of selecting a cell line which expresses a therapeutic protein and method of selecting a high-titre producing clonal cell line for large scale therapeutic protein production.

The present invention provides improved devices for co-culture of cells. The devices include inserts having invertible wells that can be lowered into a well of any standard cell culture plate. A first population of cells can be cultured in the invertible wells of the inserts and a second population of cells can be cultured in the wells of a cell culture plate. Once the first population of cells attach to the invertible wells, the inserts are flipped over and placed into the wells of the cell culture plate to co-culture with the second population of cells.

The current invention reports the use of meta-tyrosine for increasing the specific productivity of a eukaryotic cell that produces/expresses a polypeptide. In the current method it is not necessary to perform a temperature-, osmolality- or pH shift or to add drugs like valproic acid or sodium butyrate to modulate the specific productivity of the cultivated cells. The method does not affect cell viability or product titer.