Described herein are methods of recellularizing an organ or tissue matrix.

Structures and methods for tissue engineering include a multicellular body including a plurality of living cells. A plurality of multicellular bodies can be arranged in a pattern and allowed to fuse to form an engineered tissue. The arrangement can include filler bodies including a biocompatible material that resists migration and ingrowth of cells from the multicellular bodies and that is resistant to adherence of cells to it. Three-dimensional constructs can be assembled by printing or otherwise stacking the multicellular bodies and filler bodies such that there is direct contact between adjoining multicellular bodies, suitably along a contact area that has a substantial length. The direct contact between the multicellular bodies promotes efficient and reliable fusion. The increased contact area between adjoining multicellular bodies also promotes efficient and reliable fusion. Methods of producing multicellular bodies having characteristics that facilitate assembly of the three-dimensional constructs are also provided.

The present disclosure describes a microfluidic chip for culturing and in vitro testing of 3D organotypic cultures. The tests may be performed directly on the organotypic culture in the microfluidic chip. The microfluidic chip includes at least one microfluidic unit which includes two fluidic compartments, such as upper and lower, separated by a permeable supporting structure, one or more access opening for the fluidic compartments, and a set of lids interchangeable with a set of insets. The permeable support structure serves as a support for the organotypic culture. The upper and lower compartments may include inlets and outlets which allow fluids to be perfused into the lower compartment and fluids to be perfused into the upper compartment. The access opening may be closed with a lid or accommodate an inset.

Disclosed are a spheroid array and more particularly, a droplet trapping structure array capable of isolating all or selected spheroids into an isolated droplet array environment and the use thereof. The droplet-trapping structure array and the method and device for transferring spheroids using the same have the advantages of transferring droplets or spheroids with very high efficiency and very small variation between users by simply contacting two arrays. The spheroid transfer method and device enable mass-production of spheroid arrays in an isolated environment. In particular, the droplet trapping structure array and the spheroid transfer method can be useful for the treatment of spheroids with various reagents and the exchange of culture media.

Provided are engineered meat products formed as a plurality of at least partially fused layers, wherein each layer comprises at least partially fused multicellular bodies comprising non-human myocytes and wherein the engineered meat is comestible. Also provided are multicellular bodies comprising a plurality of non-human myocytes that are adhered and/or cohered to one another; wherein the multicellular bodies are arranged adjacently on a nutrient-permeable support substrate and maintained in culture to allow the multicellular bodies to at least partially fuse to form a substantially planar layer for use in formation of engineered meat. Further described herein are methods of forming engineered meat utilizing said layers.

The present disclosure provides a method of bioprinting a 3-D structure comprising one or more biologically-relevant materials on a super-hydrophobic surface. In one embodiment, the method comprises providing a composition having one or more biologically-relevant materials dispersed within a biocompatible medium. A pattern comprising a hydrophilic material is deposited on a defined area of the super-hydrophobic surface, wherein the pattern is modeled after a biological structure. The composition having the one or more biologically-relevant materials is then bioprinted atop the hydrophilic surface to form a 3-D structure, wherein the hydrophilic surface maintains the 3-D structure in a desired position or shape on the super-hydrophobic surface.

The invention relates to methods for the isolation of non-haematopoietic tissue-resident lymphocytes, particularly γδ T cells. Such γδ T cells include non-Vδ2 cells, e.g. Vδ1, Vδ3 and Vδ5 cells and such non-haematopoietic tissues include skin and gut. It will be appreciated that such isolated non-haematopoietic tissue-resident lymphocytes find great utility in adoptive T cell therapies, chimeric receptor therapies and the like. Also provided are methods for expanding isolated tissue-resident lymphocytes, particularly methods for isolating and expanding γδ T cells. The present invention also relates to both individual cells and populations of cells produced by the methods described herein.

A system includes a placement head with at least one printing nozzle configured to pick up and detachably hold at least one biological organism; an image acquisition system including a visual inspection system configured to identify at least one target biological organism to be picked up by the printing nozzle of the placement head in a first location, and identify at least one second location for deposit of the at least one target biological organism, wherein the first location is different from the at least one second location; and a robotic motion system that moves the placement head, based on input from the visual inspection system and a distance identification system, from the first location to the at least one second location, such that the placement head deposits the target biological organism at the at least one second location.

The present invention relates to a cell culture scaffold, and provides a cell culture scaffold which has a hydrogel structure comprising alginate and cellulose extracted by means of algae decellularization and which enable the stable growth of cells even at low cost while having a simple preparation.

A three-dimensional porous growth surface (carriers) made by multiple layers of netting or mesh, especially large dimension and area of fabrics that are capable to form a column-type fixed bed by rolling the layers or other shape of fixed bed by stacking or randomly disposed packing the carriers to form a packed-bed for cell culture the layers together. A method to enhance the cell growth by consistent pore dimension and structure through the application of nettings or meshes. A method of use for enhancing the cell recovery with limited layer of the netting or meshes that has fewer obstacles than other carriers made by non-woven fabrics, or porous structure materials. A method of sealing the surrounding of the multilayer nettings or meshes to reduce particle generation during cell harvest, or ease of separation by filtration due to larger wall dimension on the nettings or meshes than cells. The fixed bed make by large dimension of the growth surface can easily to manufacture a fixed bed simply by rolling the multiple layers of sheets, which can reduce the manufacture cost and also facilitate mass production of carriers for fixed bed bioreactors.