A group of inventions relates to biotechnology. Disclosed are a printing head and a printing device with fabric spheroids. The printing head includes a system of channels containing input and output channels, upper and lower channels for separation of nutrient medium, as well as a channel for a supporting plunger. Inlet channel has diameter providing movement of spheroid on it only by one. Output channel has diameter not less than diameter of inlet channel, includes input hole for input of printing tool and outlet hole for output of spheroids. Separation channels are made with possibility of connection of nutrient removal device with outlet channel through system of microchannels. Device includes a printing head, a device for feeding spheroids with a nutrient medium into an inlet channel, a device for removal of nutrient medium, a supporting plunger, a printing tool, a system for recording position of the spheroid in the output channel and a computing device for control. Inventions provide higher accuracy of positioning of spheroid on printed surface, improved quality of production of three-dimensional structures and faster printing.

The invention is directed to a modified polysaccharide hydrogel, comprising a low molecular weight alginate with a specific M/G ratio. The modified polysaccharide is modified with a specific peptide, preferably comprising a cell-adhesion peptide. The modified polysaccharide hydrogel may be used as a hydrogel for the growth of cultured meat preferably as a sacrificial biopolymer.

Provided are a scaffold including a plant protein and in-vitro meat produced by using the scaffold. Considering that the scaffold consists of a plant protein, cultured muscles or adipose tissues may be ingested together with the scaffold without being separated therefrom. By adjusting a ratio of a muscle cell and an adipocyte, in-vitro meat having desirable texture may be produced, and since various types of cells may adhere, proliferate, and differentiate on the scaffold, such a scaffold may be effectively utilized for mass production of in-vitro meat.

Described herein are bioink compositions, which may have an elastic modulus similar to a natural tissue and/or tunable mechanical properties, along with methods of preparing and using the compositions. The compositions described herein may be useful as a medium for cell and/or tissue culture and/or for bioprinting, but are not limited thereto.

An interference image acquisition apparatus includes a light source, a beam splitter, a second reflection mirror, an imager, and a first reflection mirror. A cell is placed on one side of a transparent material, and the first reflection mirror is placed on the other side of the transparent material. In a two-beam interferometer, an optical path difference between an optical path length of a first light beam reflected by the first reflection mirror and an optical path length of a second light beam reflected by the second reflection mirror is set to a coherence length of light output from the light source or less. The imager acquires an interference image in a state in which the cell is placed at a position conjugate to an imaging plane in a first optical system between the imaging plane and the first reflection mirror.

This disclosure provides novel anionic amphiphilic β-hairpin peptides that self-assemble under appropriate conditions to form a reversible gel-sol hydrogel that can be used, for example, to readily deliver protein therapeutics and cells by injection to a target location in a subject.

The present invention relates to novel compositions and methods to produce 3D organ equivalents of the brain (i.e. “mini-brains”). The invention also relates to methods of using human induced pluripotent stem cells, a combination of growth and other soluble factors and gyratory shaking. Cells from healthy or diseased donors or animals can be used to allow testing different genetic backgrounds. The model can be further enhanced by using genetically modified cells, adding micro-glia or their precursors or indicator cells (e.g. with reporter genes or tracers) as well as adding endothelial cells to form a blood-brain-barrier.

Provided herein are a scaffold having a surface hyperboloid structure and its fabrication method and application. The scaffold has internally disposed with pores where each of the pores connects with each other and any point on a surface of each of the pores has the hyperboloid structure. Since the surface of the scaffold is smooth and stress concentration is thereby avoided, the scaffold can withstand a greater external force in the case of the same porosity. Moreover, since the pores inside the scaffold connect with each other, the scaffold has a better permeability to fluid and is more conducive to tissue ingrowth. In addition, the scaffold has a large internal surface area, rendering it feasible to subsequent surface treatment, such as film coating, to be carried out on the internal surface of the scaffold.

Biomaterials for tissue regeneration and engineering applications and methods of making and use thereof are described, as well as constructs and kits derived from the biomaterials. The biomaterials can be derived from extracellular matrix and functionalized to make them crosslinkable and amenable to tuning of their material properties.

Disclosed is a method for controlling the Young’s modulus of a three-dimensional tissue containing cells and an extracellular matrix by adjusting the average diameter of an extracellular matrix in production of the three-dimensional tissue.