Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Methods are provided herein for selectively killing senescent cells and for treating senescence-associated diseases and disorders by administering a senolytic agent. Senescence-associated diseases and disorders treatable by the methods using the senolytic agents described herein include cardiovascular diseases and disorders associated with or caused by arteriosclerosis, such as atherosclerosis; idiopathic pulmonary fibrosis; chronic obstructive pulmonary disease; osteoarthritis; senescence-associated ophthalmic diseases and disorders; and senescence-associated dermatological diseases and disorders.

Provided herein are methods of reducing or eliminating undifferentiated pluripotent stem cells, where the methods comprise contacting an effective amount of a compound to a heterogeneous cell population or sample comprising or suspected of comprising differentiated cell types and undifferentiated pluripotent stem cells, whereby the contacting selectively reduces or eliminates undifferentiated pluripotent stem cells from the cell population or sample. Also provided are methods for obtaining a population of stem cell-derived cell types substantially free of undifferentiated pluripotent stem cells as well as isolated populations of such of stem cell-derived cell types.

The present invention relates to a method of screening highly efficiently stem cells using the protein marker GRP78, and more particularly, to a method of removing old stem cells with decreased expression of GRP78 and isolating only highly efficient stem cells. The use of the protein marker GRP78 according to the present invention makes it possible to isolate only highly efficient stem cells by removing old stem cells. Thus, the protein marker GRP78 may be used as an effective marker for controlling the quality of stem cell therapy products and evaluating the stability and effectiveness thereof, and is useful in the production of stem cell therapy products having excellent therapeutic efficacy.

The present invention relates to a culture method of NK cell, the method comprising: 1) isolating a pellet of peripheral blood mononuclear cell (PBMC) containing immune cells from blood by using ficoll and centrifugation; 2) culturing the isolated PBMC in an anti-CD16 antibody-coated incubator in a medium containing RPMI culture medium, plasma, IL-2, IL-15, anti-CD56, anti-NKp46, and anti-NKp30; 3) subculturing the cultured PBMC in RPMI medium, albumin, IL-2, IL-15, anti-CD56, anti-NKp46, and anti-NKp30; and 4) treating the subcultured PBMC in a medium composition based on RPMI to which anti-NKp30, anti-NKp46 and anti-CD56 antibodies are added with a cytokine selected from a cytokine group consisting of IL-2, IL-15, and IL-2+IL-15 to activate NK cells. The present invention is an optimal combination that can effectively activate cells when NK cells are treated with IL-2 and IL-15 among various cytokines, and when treated with IL-2+IL-15, mass culture of NK cells is possible. Since apoptosis or killing ability of cancer cells can be promoted when this is used, the present invention can be used as an effective immune cell therapy for preventing or treating cancer.

Disclosed in the present application is a composition for removing phospholipids and cell debris, comprising an alcohol, an alcohol ether, and a surfactant, wherein the alcohol accounts for 50-90 wt %, preferably 60-80 wt %; the alcohol ether accounts for 5-40 wt %, preferably 10-20 wt %; the surfactant accounts for 0.1-5 wt %, preferably 1-3 wt %. The present application further provides a method for removing phospholipids and cell debris on biological tissue. The composition of the present application is used for treating biological tissue, and the treated biological tissue has an excellent anti-calcification effect.

An object of the present invention is to provide an effective method for producing a population of NK cells for cell therapy. Another object of the present invention is to improve in vitro amplification efficiency of NK cells. A still another object of the present invention is to flexibly increase signals required for licensing of NK cells. There is provided a method for producing a cell population including NK cells, which comprises preparing a cell population of mononuclear cells originating in a plurality of donors and including NK cells, and incubating the prepared population of mononuclear cells under conditions effective for treating and proliferating NK cells to proliferate NK cells.

A method of killing specific cells from among a group of cells cultured in a culture vessel by quick and brief laser treatment, the cell culture vessel comprising a main body and a to-be-irradiated layer attached to the main body, the to-be-irradiated layer containing an ingredient capable of absorbing laser light upon laser irradiation, the group of cells being cultured on the surface of the to-be-irradiated layer, the method comprising: applying laser light to a partial area of the to-be-irradiated layer directly below the specific cells.

Disclosed is a method for producing a high-quality graft from pluripotent stem cell-derived differentiation-induced cells, a graft produced by using the method, and a method for treating a disease using the graft are described Embodiments of a method for producing a graft are described, which include a step of performing an operation for removing undifferentiated cells in a cell population containing pluripotent stem cell-derived differentiation-induced cells, optionally a step of freezing the cell population and thereafter thawing the cell population, and a step of seeding the obtained cell population on a culture substrate and performing graft-forming culture.

Provided are a method and an apparatus for isolating stromal cells from biological tissue without using an enzyme, in which spontaneous migration of the stromal cells of the biological tissue is induced to move the stromal cells to the outside of the biological tissue, wherein the induction of the spontaneous migration of the stromal cells is performed in a state in which the biological tissue is attached to an attachment member formed of a material to which biological tissue is attachable, and is performed in a culture medium in which the stromal cells can survive.