Provided are a method for preparing a liquid-state dripping or coating pathological quality control product, and uses thereof. The method comprises: selecting and determining a control with a control value, and processing the control; adding an ethanol solution to the processed control for preserving for standby use, with the amount of the ethanol solution added depending on the amount of a precipitate; and performing setting of a positive or negative control by means of dripping or smearing. The pathological quality control product is a suspension or homogenate of micro tissue sections, cell/cultured cell sections, or cultured cells. Another aspect of the present invention provides a use of the liquid-state dripping or coating pathological quality control product as a positive or negative control in immunohistochemistry, in situ hybridization, special staining and other tissue staining detection, or as a standard quality control product for pathological internal quality control and external quality control.

Described herein are methods for selecting lymphocytes for adoptive cell therapy based on P-glycoprotein expression and compositions comprising same.

A novel class of agents has been identified to serve as cell-guard agents and/or target-specific supplements to increase cell quality and yield, as well as select for target cell populations. Several additive agents (both natural and synthetic) have been identified, including Vitamin D3, NAC, resveratrol, salubrinal, AKT, and tunicamycin (among others) that hold promise for application in cell models. In one embodiment, hypothermic stress regimes are utilized. In another embodiment, normothermic conditions are utilized while other stressors are tested in the processing. The methods of maintaining mass cell cultures and/or selecting out particular cell populations for further research and clinical use represents an important step in therapeutic discovery.

A fluid circuit for cell washing is provided that comprises a spinning membrane separator and a fluid management system comprising a cassette that defines the fluid pathways, and including internally mechanical valving, pressure sensing and air sensing for controlling flow through the fluid pathways, thus minimizing the volume of the fluid circuit. Additionally, the fluid circuit comprises syringes that are acted on by syringe pumps associated with the hardware component of the system to provide pressure for moving fluid through the circuit. Preferably, the syringes are connected directly to the cassette, or formed integrally within the cassette housing, thus further minimizing the volume of the fluid circuit.

A method and apparatus associated with aseptic processing and production of cells in a non-sterile enclosure apparatus without chemical biocides is disclosed, by controlling the level of humidity throughout the enclosure to 25% relative humidity (RH) or less, and preferably at 20% or 15% or less RH. In addition, the temperature is controlled to 37 C., and continuous gas flow is maintained in the enclosure. Colony forming units from microbial contamination detected by environmental monitoring within the enclosure are significantly reduced.

A laser processing machine for killing specific cells from a group of cells on a surface of a layer containing an ingredient capable of absorbing laser light, the laser processing machine being configured to: control a laser light source to output laser light at 5W or less and at a wavelength of 380 nm or greater such that the laser light source is applied to a second area on a second surface of the layer opposed to the first surface; and control an actuator to provide a relative movement between the second area where the laser light is applied and the layer at a rate of 2000 mm/sec or less such that the irradiated second area absorbs energy to generate heat that kills unwanted cells on a first area of the first surface and the laser light does not instantly kill the specific cells on the first area upon irradiation.

The present invention provides a cell detachment agent for detaching a cell to be separated from a separation carrier bound to the cell to be separated, wherein the above-mentioned cell detachment agent contains a bindable ureido group-containing polymer having a side chain structure represented by the following formula (1) and having a separation carrier-binding site capable of binding to the above-mentioned separation carrier at a side chain or terminal thereof, and the above-mentioned bindable ureido group-containing polymer has an upper critical solution temperature (UCST) of 4 to 40? C.:

NH(C?O)NH.sub.2(1)

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

The present invention relates to antibodies targeted to BDCA2 that deplete plasmacytoid dendritic cells (pDC) and methods of using the antibodies to treat disorders associated with pDC.

The invention is directed to a Perfusion device for biological tissues comprising a casing having two parts, a first part (1) and a second part (9), a holder (7) for a plurality of hollow penetration structures (8), wherein the hollow penetration structures (8) are provided with at least one orifice having fluid communication through the holder (7) a support (5) for the biological tissue (6) characterized in that the support (5) for the biological tissue (6) is positioned in the casing at a distance to the holder (7) that by joining the first part (1) and the second part (9) to form the casing, the hollow penetration structures (8) are in proximity to the holder (7). Use of the perfusion device in a process for disaggregation of a biological tissue to yield target cells.