The present disclosure provides a method for promoting the growth of parietal decidual basalis-mesenchymal stem cells (PDB-MSCs), which comprises promoting the growth of parietal decidual basalis-mesenchymal stem cells (PDB-MSCs) via a human umbilical cord-mesenchymal stem cell (UC-MSC)-derived exosome. In the present disclosure, after the PDB-MSCs are co-cultivated with the human UC-MSC-derived exosome, the PDB-MSCs show strong cell proliferation ability, prominent cell shape, and desirable cell viability. That is, the human UC-MSC-derived exosome of the present disclosure can improve a quality of PDB-MSCs and effectively improve the ability of PDB-MSCs to secrete vascular endothelial growth factor (VEGF) and stem cell factor (SCF), so as to solve the problem that PDB-MSCs show decreased proliferation ability and poor cell viability after multiple passages, which effectively facilitates the large-scale cultivation and clinical practice of PDB-MSCs.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

The present invention relates to apparatuses for use in electrophoretic separation of macromolecules and/or cells, and in which circulating buffer streams are not required for the electrophoretic separation of macromolecules and/or cells. In certain embodiments, the electrophoretic apparatus disclosed herein comprise a sample chamber and a harvest chamber separated by a size-exclusion membrane; non-circulating buffer chambers flanking each respective sample chamber and harvest chamber, wherein each buffer chamber is separated from each respective sample chamber and harvest chamber by an ion-permeable membrane (restriction membrane), and wherein the buffer chambers are sealed and contain a buffer solution; and an electrode positioned in each buffer chamber. Also disclosed are related methods of using the electrophoretic apparatus disclosed herein.

A potent short hairpin RNA (shRNA734) directed to human Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) improves the rate of gene-modified stem cell engraftment by a conditioning and in vivo selection strategy to confer resistance to a clinically available guanine analog antimetabolite, 6TG, for efficient positive selection of gene-modified stem cells. Uses for polynucleotides comprising the shRNA734 include methods for knocking down HPRT in a cell, for conferring resistance to a guanine analog antimetabolite in a cell, for producing selectable genetically modified cells, for selecting cells genetically modified with a gene of interest from a plurality of cells, for removing cells genetically modified with a gene of interest from a plurality of cells, and for treating a subject infected with HIV.

The present invention provides a method for selecting for cells transduced to express a nucleic acid sequence of interest (NOI), which comprises the following steps: (a) transducing a population of cells with a vector co-expressing the NOI and a nucleic acid sequence which inhibits Fas expression or activity in the cell; (b) exposing the cells from (a) to FasL such that untransduced cells are eliminated by apoptosis. The present invention also provides a molecule which comprises a Fas-binding domain linked to an intracellular retention signal which may be used in such a method to inhibit Fas expression.

The present invention provides a method for separating a stromal vascular fraction (SVF) from adipose tissue by using a non-contact ultrasonic device comprising a power supply unit, an ultrasonic oscillation unit, and an ultrasonic vibration unit; and a non-contact ultrasonic device used therefor. Specifically, the present invention provides a method for separating a stromal vascular fraction (SVF) from adipose tissue, and a non-contact ultrasonic device used therefor, in which the stromal vascular fraction can be extracted from adipose tissue with high efficiency while minimizing cell contamination and destruction by using a non-enzymatic method and a non-contact ultrasonic device.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

Provided is a cell population comprising differentiated cells obtainable by inducing differentiation of pluripotent stem cells, wherein the content ratio of undifferentiated pluripotent stem cells is 0.2% or less.

Methods of expanding peripheral blood lymphocytes (PBLs) from blood of patients with hematological malignancies, including lymphomas and leukemias, genetic modifications of expanded PBLs to incorporate chimeric antigen receptors, genetically modified T cell receptors, and other genetic modifications, and uses of such expanded and/or modified PBLs in the treatment of diseases such as cancers and hematological malignancies are disclosed herein.

The present invention concerns enriched heterogeneous mammalian renal cell populations characterized by biomarkers, and methods of making and using the same.