The present disclosure describes a DNA fragment mixture for the prevention or for the treatment of at least one pathogenic, parasitic or infesting species of plants or of the environment, wherein the DNA mixture consists of random fragments of total DNA of at least one pathogenic, parasitic, or infesting species, and/or at least one phylogenetically similar species, against which the prevention and treatment are directed. Further, the disclosure describes a process and related system for improvement of the production/growth of microorganisms at high yield in bioreactors or photobioreactors, or of plants in different culture systems, where the nucleic acids of the organisms produced/grown by such a process are removed from the culture medium and the culture medium, deprived of these nucleic acid, is used again in the process.

Numerous plant microbes, including the vascular-limited Candidatus spp.—causal agents of citrus greening and potato zebra chip diseases—are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system (e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.

Numerous plant microbes, including the vascular-limited Candidatus spp.—causal agents of citrus greening and potato zebra chip diseases—are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system (e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.

Disclosed are a method of promoting hair growth or preventing hair loss in a mammal. In one aspect, the method includes administering interdermally or subcutaneously an agent containing an active ingredient comprising bFGF and a pharmaceutically acceptable substance, wherein bFGF is administered in a dose of 1 μg to 100 μg per 1 cm.sup.2 of the target area of hair growth treatment or hair loss prevention and a composition for use in the method.

The invention relates to the soybean variety designated 01058741. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01058741. Also provided by the invention are tissue cultures of the soybean variety 01058741 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01058741 with itself or another soybean variety and plants produced by such methods.

The invention relates to the soybean variety designated 01058771. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01058771. Also provided by the invention are tissue cultures of the soybean variety 01058771 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01058771 with itself or another soybean variety and plants produced by such methods.

Plants are provided with increased ribulose-1,5-bisphosphate (RuBP) regeneration capacity during the Calvin cycle through increased expression of sedoheptulose 1,7 bisphosphatase, in combination with reduced photo-respiratory losses through expression of glycolate catabolizing enzymes. Such plants have a greater growth rate and/or improved biomass and/or increased carbon fixation compared to untreated plants, or plants comprising only one of the features above.

The system includes a pre-fabricated garden seedsheet, with seeds and soil embedded in water-soluble pods within a weed barrier fabric. Each water-soluble pod is fabricated from dissolvable material, which are affixed together to form cavities in which seeds and soil are applied. The pods are then affixed to the weed barrier matrix in an arrangement dictated by the plant algorithm. Upon contact with water, the pods rapidly dissolve, thereby beginning the germination process in which the seeds sprout and emerge through the aligned openings in the weed barrier fabric. The selection of plants and their physical arrangement within each seedsheet may be determined by a software-driven plant algorithm that extrapolates plant characteristics, environmental requirements, and companion benefits. The algorithm may be used both on the customer-facing software program, as well as the in-house design process to create pre-designed seed sheets.

The system includes a pre-fabricated garden seedsheet, with seeds and soil embedded in water-soluble pods within a weed barrier fabric. Each water-soluble pod is fabricated from dissolvable material, which are affixed together to form cavities in which seeds and soil are applied. The pods are then affixed to the weed barrier matrix in an arrangement dictated by the plant algorithm. Upon contact with water, the pods rapidly dissolve, thereby beginning the germination process in which the seeds sprout and emerge through the aligned openings in the weed barrier fabric. The selection of plants and their physical arrangement within each seedsheet may be determined by a software-driven plant algorithm that extrapolates plant characteristics, environmental requirements, and companion benefits. The algorithm may be used both on the customer-facing software program, as well as the in-house design process to create pre-designed seed sheets.

Stevia varieties with high a content of RebD, a high content of RebM, and a high content of RebD and RebM containing various SNP markers and UGT isoforms, are disclosed. Methods of screening for the SNPs are also disclosed as well as for using the SNPs in marker assisted breeding. Further provided are methods for introgressing the disclosed SNPs associated with high RebD and high RebM into stevia plants by selecting plants comprising for one or more SNPs and breeding with such plants to confer such desirable agronomic phenotypes to plant progeny.