A seed cell medium of a tumor-infiltrating lymphocyte and an application thereof. The medium contains a cell culture component, a cell factor, and an immune checkpoint antibody or an antigen-binding fragment thereof. The cell factor includes IL-2; an immune checkpoint includes PD-1, LAG-3, TIGIT, and/or CTLA-4; and the cell culture component is a serum medium or a serum-free medium. The seed cell medium accelerates a culture of a seed cell of the tumor-infiltrating lymphocyte and shortens the amplification time required by the tumor-infiltrating lymphocyte.

Disclosed is a method for detecting chemotaxis of neutrophil, including: mixing a blood sample and an equal volume of glucose, mixing, and standing; taking a supernatant of the blood sample after standing into a centrifuge tube, and centrifuging; adding 1× calcium and magnesium ion-free HBSS into a centrifuged blood sample to re-suspend cell aggregates at bottom; uniformly blowing and dispersing a re-suspended bottom cell aggregates, and slowly adding a polysucrose solution from the bottom of the centrifuge tube to perform density gradient centrifuging treatment; dividing a density gradient centrifuged solution into three layers, pipetting a clear liquid at an upper layer and a PBMC layer at a middle layer to obtain cell aggregates at a bottom layer; lysing erythrocytes in the cell aggregates, then successively adding 2× calcium and magnesium ion-free HBSS and 1× calcium and magnesium ion-free HBSS, mixing, and centrifuging again to obtain neutrophils.

Provided are cells containing exogenous antigen and uses thereof.

The present invention relates to exosomes isolated from dermal papilla progenitor cells, specifically, the exosomes isolated from the dermal papilla progenitor cells which are excellent in prevention, improvement and treatment of hair loss (alopecia) and are also excellent in terms of skin improvement and wound healing effects, as well as various uses thereof.

There is provided a quality improving agent for iPS cells, including a polynucleotide, in which the polynucleotide contains an H1foo gene and a regulatory sequence that is capable of regulating at least one of the amount and the period of existence of an H1foo protein expressed from the H1foo gene in cells when the H1foo gene is transduced into the cells.

Provide are a primay cell culture medium that contains an MST1/2 kinase inhibitor and is used for culturing laryngeal cancer epithelial cells, and a culture method using the primary cell culture medium. In the culture method, the primay cell culture medium is used to culture primary cells on a clulture vessel plated with irradiated trophoblasts, so that the primay cells proliferate rapidly. A cell model obtained by the primary cell culture medium and the primay cell culture method can be used for the efficacy evaluation and screening of drugs.

A method for the mass proliferation of urine-derived multipotent cells and a medium composition for the mass proliferation of urine-derived multipotent cells according to the present invention can be used to massively proliferate urine cells by efficiently isolating the same even from urine that has been left alone for a long period of time, and can be used to produce multipotent cells having characteristics of epithelial cells, mesenchymal cells, and stem cells.

A cell transplanting kit includes a transplant including a cell group and a cell transplanting device. The cell transplanting device includes a needle-shaped portion that extends in a shape of a tube, and an aspiration portion configured to aspirate an interior of the needle-shaped portion. The needle-shaped portion is configured to attract the transplant by aspiration by the aspiration portion and take the transplant into the interior. The aspiration portion is configured to create an aspiration pressure in a range of −100 kPa to −0.1 kPa. The transplant includes a protection portion in a form of a gel covering at least a part of the cell group. The transplant has an outer diameter that is greater than or equal to a minimum value of the inner diameter of the needle-shaped portion. The protection portion has a jelly strength of greater than or equal to 100 g.

The present invention relates to the preparation of mesenchymal stem cells, which are for the proliferation of viral vectors and viruses, and enable virus production and release timing control such that tumor targeting can be improved and viruses can be mass-produced. In addition, the tumorigenesis of mesenchymal stem cells of the present invention can be fundamentally blocked, safety is secured by enabling virus production and release to be controlled at desired time points, and antitumor effects can be maximized.

An access system having a communication component that interfaces with a first device and a second device, where the first device is located inside or on an entity and coupled to a biological organism of the entity, and where the second device is located outside the entity and a controller component that controls a function of the first device, employing the communication component, to provide treatment to the biological organism of the entity coupled to the first device based on a request received from the second device.