The present disclosure relates to genetically engineered host cells and methods of producing a lipid-derived compound by employing such host cells. In particular embodiments, the host cell includes a first mutant gene encoding a cytoplasmic tRNA thiolation protein. Optionally, the host cell can include other mutant genes for decreasing fatty alcohol catabolism, decreasing re-importation of secreted fatty alcohol, or displaying other useful characteristics, as described herein.

Provided herein are methods for generating cellular biomass in continuous aerobic fermentation systems. The biomass yield, and the concentration of polyhydroxyalkanoate within the biomass, are each directed to advantageous levels by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are biomass produced using the provided methods, and animal feed compositions including the provided biomass.

Provided herein are methods for generating cellular biomass in continuous aerobic fermentation systems. The biomass yield, and the concentration of polyhydroxyalkanoate within the biomass, are each directed to advantageous levels by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are biomass produced using the provided methods, and animal feed compositions including the provided biomass.

Provided are a taxadiene synthase TcTS2, an encoding nucleotide sequence and use thereof. The amino acid sequence of TcTS2 includes or consists of: (a) an amino acid sequence represented by SEQ ID NO: 1; or (b) a functional homologous sequence having at least 80% sequence similarity with the amino acid sequence represented by SEQ ID NO: 1; or (c) an amino acid sequence having TcTS2 activity with addition, deletion, or substitution of one or more amino acids in the amino acid sequence represented by SEQ ID NO: 1. TcTS2 and the nucleotide sequence encoding the TcTS2 provide new gene resources for improving the yield of taxol, and they may be used for modifying chassis hosts by plant genetic engineering and metabolic engineering strategies to produce taxol and the intermediates thereof etc., thereby having significant economic and social value.

Provided are a taxadiene synthase TcTS2, an encoding nucleotide sequence and use thereof. The amino acid sequence of TcTS2 includes or consists of: (a) an amino acid sequence represented by SEQ ID NO: 1; or (b) a functional homologous sequence having at least 80% sequence similarity with the amino acid sequence represented by SEQ ID NO: 1; or (c) an amino acid sequence having TcTS2 activity with addition, deletion, or substitution of one or more amino acids in the amino acid sequence represented by SEQ ID NO: 1. TcTS2 and the nucleotide sequence encoding the TcTS2 provide new gene resources for improving the yield of taxol, and they may be used for modifying chassis hosts by plant genetic engineering and metabolic engineering strategies to produce taxol and the intermediates thereof etc., thereby having significant economic and social value.

Processes for preparing and purifying protein from plant material, and compositions and uses comprising the same, are provided.

Processes for preparing and purifying protein from plant material, and compositions and uses comprising the same, are provided.

Transgenic plants with blue flower color, or their inbred or outbred progeny, or their propagules, partial plant bodies, tissues or cells, are provided. A buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog is transferred into a host plant to cause delphinidin-type anthocyanins and flavone mono-C-glycosides to be copresent in the plant cells.

Transgenic plants with blue flower color, or their inbred or outbred progeny, or their propagules, partial plant bodies, tissues or cells, are provided. A buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog is transferred into a host plant to cause delphinidin-type anthocyanins and flavone mono-C-glycosides to be copresent in the plant cells.

Provided is a polypeptide tag. The amino acid sequence of the polypeptide tag is Xaa1Xaa2Xaa3PHDYNXaa4Xaa5Xaa6 (SEQ ID NO: 37), wherein in the formula, Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, and Xaa6 are each independently an amino acid or none. The polypeptide tag is used for labeling a target protein. In a second aspect, provided is a polypeptide fusion protein, comprising the following two structures: (1) any polypeptide tag according to the first aspect, and (2) a target protein connected to the polypeptide tag. Also provided are an in vitro cell-free protein synthesis system and an application thereof in in vitro protein synthesis. By constructing the polypeptide tag and a target protein as a fusion protein, the expression of the labeled target protein can be effectively increased without removing the polypeptide tag.