Materials and methods are provided for making plants (e.g., Nicotiana varieties) that are suitable for producing therapeutic polypeptides suitable for administration to humans and animals, particularly by making TAL effector endonuclease-induced mutations in genes encoding xylosyltransferases and fucosyltransferases.

Materials and methods are provided for making plants (e.g., Nicotiana varieties) that are suitable for producing therapeutic polypeptides suitable for administration to humans and animals, particularly by making TAL effector endonuclease-induced mutations in genes encoding xylosyltransferases and fucosyltransferases.

The present invention provides compositions and methods for regulated gene expression. In certain aspects, the invention relates to an inducible synthetic promoter that can be used for regulated gene expression or to generate mutations in one or more bacterial cells of the gut microbiota.

A neutron ray irradiation target apparatus 100 of the present invention is used to irradiate irradiation targets (seeds, etc.) with a neutron ray generated by a neutron ray irradiation apparatus. The neutron ray irradiation target apparatus 100 has a holding means 70 for holding the irradiation targets. The holding means 70 holds at least one closed container 30 which can accommodate the irradiation targets 20 stacked randomly and three-dimensionally. In the case where the irradiation targets are stacked three-dimensionally and accommodated in the closed container, the irradiation targets overlapping each other are irradiated with the neutron ray in a chain reaction fashion. The neutron ray irradiation target apparatus 100 can be used in a method for irradiating a large amount of irradiation targets (seeds of crops, etc.) with a neutron ray, while reducing a required time, thereby efficiently inducing mutations in the irradiation targets.

A neutron ray irradiation target apparatus 100 of the present invention is used to irradiate irradiation targets (seeds, etc.) with a neutron ray generated by a neutron ray irradiation apparatus. The neutron ray irradiation target apparatus 100 has a holding means 70 for holding the irradiation targets. The holding means 70 holds at least one closed container 30 which can accommodate the irradiation targets 20 stacked randomly and three-dimensionally. In the case where the irradiation targets are stacked three-dimensionally and accommodated in the closed container, the irradiation targets overlapping each other are irradiated with the neutron ray in a chain reaction fashion. The neutron ray irradiation target apparatus 100 can be used in a method for irradiating a large amount of irradiation targets (seeds of crops, etc.) with a neutron ray, while reducing a required time, thereby efficiently inducing mutations in the irradiation targets.

Provided herein are methods and compositions for increasing fetal hemoglobin levels in a cell by disrupting BCL11A expression at the genomic level. Also provided herein are methods and compositions relating to the treatment of hemoglobinopathies by reinduction of fetal hemoglobin levels.

Provided herein are methods and compositions for increasing fetal hemoglobin levels in a cell by disrupting BCL11A expression at the genomic level. Also provided herein are methods and compositions relating to the treatment of hemoglobinopathies by reinduction of fetal hemoglobin levels.

Provided herein are systems, compositions, and methods for the incorporation of unnatural amino acids into proteins via nonsense suppression or rare codon suppression. Nonsense codons and rare codons may be introduced into the coding sequence of a protein of interest using a CRISPR/Cas9-based nucleobase editor described herein. The nucleobase editors are able to be programmed by guide nucleotide sequences to edit the target codons in the coding sequence of the protein of interest. Also provided are application enabled by the technology described herein.

The present invention relates to methods and compositions for modifying a target site in the genome of a cell. Fusion proteins including one or more DNA binding domains and one or more heterologous domains, such as DNA modifying domains, connected by improved linker sequences are provided. Codon optimized polynucleotides encoding fusion proteins including one or more DNA binding domains and one or more heterologous domains connected by improved linker sequences are provided.

The present invention relates to methods and compositions for modifying a target site in the genome of a cell. Fusion proteins including one or more DNA binding domains and one or more heterologous domains, such as DNA modifying domains, connected by improved linker sequences are provided. Codon optimized polynucleotides encoding fusion proteins including one or more DNA binding domains and one or more heterologous domains connected by improved linker sequences are provided.