Provided are thiol-acrylate hydrogels and tunable cell culture materials including thiol-acrylate hydrogels, and methods of making thereof. Also provided are systems for forming three-dimensional cell culture scaffolds including the materials, and methods of culturing cells, including cancer cells, using thiol-acrylate hydrogels and tunable cell culture materials. The materials herein can be used in microfluidic droplet-generating devices.

The present invention discloses a method for producing γ-aminobutyric acid (GABA) by fermentation of native strain from raw material of Sayram Ketteki, comprising the following steps: dissolving the cow's milk in the normal saline for dilution to obtain diluents with different gradients first, coating the diluents in solid media for culture respectively, isolating single colonies and storing them in a glycerin cryopreservation tube for cryopreservation respectively; next, inoculating them in liquid medium for culture respectively, and inoculating them in fermentation medium for culture for culture after activation. The content of GABA product obtained by the present invention can reach 450 μg/mL.

The present invention concerns a process for producing enzymes by a strain belonging to a filamentous fungus, said process comprising two steps: (a) a first step of growing the fungi, in the presence of at least one carbon-based growth substrate, in a stirred and aerated bioreactor in batch phase, at a pH of not more than 4.6; (b) a second step of producing enzymes, starting from the culture medium obtained in the first step (a), in the presence of at least one inductive carbon-based substrate, at a pH of not more than 4.6.

The present invention relates to streptococcal vaccine formulations and their use in generating immunity against streptococcal infection.

The present disclosure provides methanotrophic bacteria that are modified to produce less glycogen, and methods of using the modified methanotrophic bacteria to produce a desired product, such as protein(s) or metabolite(s).

The invention relates to a method for producing a mesenchymal stem cell (MSC), the method comprising culturing a primitive mesoderm cell in a mesenchymal colony forming medium (M-CFM) comprising LiCl and FGF2, but excluding PDGF, under normoxic conditions for sufficient time for a mesenchymal colony to form, and culturing the mesenchymal colony adherently to produce the MSC, wherein the MSC has superior T-cell immunosuppressive properties relative to an MSC not produced in said M-CFM. The invention also relates to an MSC produced by the method, a population of MSCs produced by the method, a therapeutic composition comprising the MSC produced by the method, an M-CFM and an M-CFM in concentrated form, and method and uses of the MSC or population in treating a disease.

Described herein are cells, cell culture methods, and cell culture media compositions useful for producing and maintaining iPSC-derided cell lines that are of higher purity and maintain cell type integrity better than current iPSC-derived cell lines. Also disclosed are methods of using the described cells and media, such as therapeutic methods of use for the described cells. The described cells include iPSC-derived mesodermal precursor cells (MPC), which itself may differentiate into at least four different cell types. When cultured under appropriate conditions, the mesodermal precursor cells can be used to produce hematopoietic stem cells (HSC), mesenchymal stem cells (MSC), smooth muscle cells (SMC), or unlimited functional endothelial cells (UFEC). One characteristic that makes the described cells desirable is that they can be maintained in culture for a number of days, or passages, without changing phenotype through differentiation.

This disclosure relates to methods of preserving cells for space exploration, methods of culturing cells, and cell growth media. In certain embodiments, methods comprise contacting cells, such as stem cells, induced pluripotent cells, progenitor cells, and cardiac associated cells, with a cell growth medium disclosed herein providing replicated cells. In certain embodiments, methods comprise preserving and culturing cells in outer space comprising; a) freezing cells providing frozen cells; b) transporting the frozen cells to outer space; c) thawing the cells providing thawed cells; and d) culturing the thawed cells with a growth medium disclosed herein.

Disclosures herein are directed to chemically defined animal-derived component free supplements designed for individual cell types that supports the ex vivo growth of cells as well or better than serum, in chemically defined conditions.

The invention provides novel methods and compositions for microspore embryogenesis and the production of doubled haploid embryos. For example, the methods provided include the steps of sterilization of pepper buds, microspore isolation from sterilized buds, liquid culture of microspores, a double-layer subculture, embryo harvest for germination and conversion to plantlets, and acclimatization of cultured plantlets.