The invention feature methods and compositions for treating hematopoietic disorders, inflammatory conditions, and cancer and providing stem cell therapy in a mammal.

The present invention relates to a method for in vivo production of seamless DNA vectors in E. coli, said seamless DNA vectors comprising a DNA sequence of interest and a phage lambda integrase recombination sequence. The method comprises providing an E. coli strain encoding a mutant phage lambda integrase (IntC3), stringently controlled by an inducible expression control sequence; transforming into the E. coli strain a bacterial plasmid comprising the DNA sequence of interest and a bacterial backbone sequence flanked by two directly repeated lambda integrase recombination sequences, wherein the bacterial backbone sequence comprises a selection marker; cultivating the transformed E. coli cells under conditions selective for the selection marker; inducing the expression of IntC3 to facilitate recombination to obtain a dimeric DNA catenane consisting of a first circular DNA molecule carrying the bacterial backbone and a second circular DNA molecule carrying the DNA sequence of interest and the phage lambda integrase recombination sequence.

A method of producing three-dimensional cellular tissues includes: obtaining a stromal cell-containing mixture containing stromal cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a stromal cell-containing mixture containing stromal cells, a cationic substance and a fragmented extracellular matrix component; gelling the stromal cell-containing mixture to obtain a first gel composition containing stromal cells; obtaining a target cell-containing mixture containing target cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a target cell-containing mixture containing target cells, a cationic substance and a fragmented extracellular matrix component; placing the target cell-containing mixture in contact with the first gel composition; gelling the target cell-containing mixture to obtain a second gel composition containing target cells; and incubating the first gel composition and the second gel composition to obtain three-dimensional cellular tissues.

The present invention provides an immunomodulatory compound comprising a carbohydrate polymer comprising mannose, wherein the carbohydrate polymer is conjugated to at least one immune modulator. The present invention also provides for the use of this compound in immunomodulatory compositions for vaccination and gene therapy methods, together with processes for its preparation.

A suspension composition for a hematology analysis control particularly useful for preserving relevant detectable characteristics of blood cells for a prolong stability period. The suspension may include at least one polysaccharide, which may include or derive from chitosan and/or chitin, as a stabilizing agent.

A method for producing an epithelial cell sheet, comprising culturing cells derived from oral mucosal epithelial cells on a substrate in a serum-free medium, wherein the serum-free medium comprises (i) EGF protein or KGF protein, (ii) B-27 supplement, and (iii) a ROCK inhibitor.

The present application provides a method for rejuvenating cells, characterized in that providing a cell to culture in a composition for a suitable period of time of time, wherein the composition is obtained by adding a chitosan solution to a cultural environment. The present application further provides a composition for rejuvenating cells, comprising a chitosan solution in a cultural environment.

Novel MSC stem-cell culture and therapy methods and culture medium compositions for the purpose of inducing, activating, or priming discrete uniform cell phenotypes to selectively promote or suppress inflammation and immunity, yielding polarized, primed, activated, or induced cells used in cell-based therapy.

Disclosed is a method for manufacturing a composite nanofiber support and a fish collagen/synthetic polymer nanofiber support manufactured by the method, wherein the method comprises: a first step for dissolving a synthetic polymer in an organic solvent; a second step for dissolving a fish collagen in water to prepare an aqueous fish collagen solution; a third step for adding the aqueous fish collagen solution prepared in the second step to the synthetic polymer solution prepared in the first step, followed by mixing; and a fourth step for electrospinning the mixture solution prepared in the third step to manufacture a nanofiber support.

Compounds and compositions comprising glycogen or phytoglycogen nanoparticles are provided that suppress type I interferon innate immune responses. The glycogen or phytoglycogen nanoparticles in the composition are suitably cationized, in one embodiment, functionalized with an amino group.