A method for preparing a bioactive composition containing conditioned cell culture medium is disclosed. The method comprises culturing cells of two or more eukaryotic cell line to form conditioned culture media, separating the cultured cells from the conditioned culture media, and combining conditioned culture media to form a bioactive composition. Novel bioactive compositions, formulations and their use in treating of a variety of diseases and health conditions are also disclosed.

Described herein are compositions and methods of forming multi-cellular tumoroids. Also described herein are methods of using the multi-cellular tumoroids.

The culture insert container has a lower culture container having a bottom portion equipped with a porous membrane and a sidewall, and having an upper insertion fixture having a cylindrical sidewall that is inserted into said lower culture container. The cylindrical sidewall has a land arranged so as to protrude from a top end of the sidewall of the lower culture container at a location such as will cause a top surface of said porous membrane and a bottom end portion of the cylindrical sidewall which is inserted within the lower culture container to be maintained in a noncontacting state. A land is arranged at an outer surface of the cylindrical sidewall so as to cause a constant distance to be maintained between an inner surface of the sidewall of the lower culture container and the outer surface of the cylindrical sidewall.

A method for preparing a bioactive composition containing conditioned cell culture medium is disclosed. The method comprises culturing cells of two or more eukaryotic cell line to form conditioned culture media, separating the cultured cells from the conditioned culture media, and combining conditioned culture media to form a bioactive composition. Novel bioactive compositions, formulations and their use in treating of a variety of diseases and health conditions are also disclosed.

An object of the present invention is to provide a new means for suppressing inhibition of proliferation of keratinocytes even when the keratinocytes start to come into contact with each other. The present invention solves the above problem by culturing keratinocytes in contact with a common medium with fibroblasts treated with a composition containing a specific compound.