Devices and systems for cell culture that include one or more hollow fibers or channels integrated into a chamber are provided. The hollow fibers or channels and/or the chamber are seeded with one or more cell types. Methods of co-culturing two or more cell types in the devices are also provided.

Hybrid suspension cultures supplementing soluble extracellular matrix (ECM) for growth of organoids is disclosed. Viable lung organoid from epithelial, endothelial, and fibroblast human stable cell lines in suspension culture are also disclosed.

A method for vascular regeneration comprises delivering endothelial cells to a lung scaffold, delivering perivascular cells to the lung scaffold, and providing a multiphase culture program to the scaffold. The multiphase culture program comprises a first phase including delivering an angiogenic medium, e.g., having 40-100 ng/ml of pro-angiogenic factors, and a second phase including delivering a stabilization medium, e.g., having 0.5-2% of serum and 1-20 ng/ml of angiogenic factors.

Disclosed are living, three-dimensional tissue constructs for in vitro scientific and medical research, arrays thereof, and methods of making said tissues and arrays.

Provided herein are artificial lung organoids. The artificial lung organoids may include an epithelial cell layer comprising mammalian lung epithelial cells, a stromal cell layer comprising mammalian lung fibroblast cells and an endothelial cell layer comprising mammalian endothelial cells. The artificial lung organoids may optionally include a porous membrane between said epithelial cell layer and said stromal cell layer and/or between said stromal cell layer and said endothelial lung cell layer.

The present invention is intended to provide a novel culture method of liver-derived cells. In other words, liver-derived cells are co-cultured with one or more types of cells selected from a group consisting of intestine-derived cells, lung-derived cells, and embryonic membrane-derived cells. Alternatively, the liver-derived cells may be cultured in the presence of the culture supernatant obtained by culturing one or more types of cells selected from the group consisting of intestinal-derived cells, lung-derived cells, and embryonic membrane-derived cells.

Provided herein include synthetic endometrial tissues and uterus-like structures for assessing embryo implantation and growth in vitro. Provided herein also include methods and devices for making the synthetic endometrial tissues and uterus-like structures and uses thereof to culture embryos (e.g., human embryos). The methods and synthetic endometrial tissues and structures generated herein can be used to culture pre-implantation embryos (e.g., human embryos) to reach a post-implantation stage.

Disclosed are living, three-dimensional tissue constructs for in vitro scientific and medical research, arrays thereof, and methods of making said tissues and arrays.

The present disclosure provides a newly-identified transitional cell state in alveolar regeneration, models to ablate lung alveolar type-1 cells that leads to lung fibrosis and emphysema, a scalable, an ex vivo lung fibrosis model that uses co-cultured lung fibroblasts and pre-alveolar type-1 transitional cell state (PATS) for the use of disease modeling and drug screening, and methods of using same.