Disclosed herein are methods for manufacturing transdifferentiated populations of non-pancreatic human insulin producing cells, and methods for enriching populations of non-pancreatic -cells for cells comprising an enriched capacity for transcription factor-induced transdifferentiation into a pancreatic -cell phenotype and function.

Methods for derivation, culture, and maturation of small hepatic progenitor cells are described.

Provided are: a method for producing biliary epithelial progenitor cells, said method comprising a step for culturing hepatoblasts in a medium containing TGF and EGF; and a method for constructing a three-dimensional duct-like structure of biliary epithelial progenitor cells, said method comprising a step for culturing hepatoblasts or biliary epithelial progenitor cells in a medium containing HGF, EGF, a Notch inhibitor and a GSK3 inhibitor in the presence of a three-dimensional scaffold material.

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same.

The present invention relates to hepatic organoids and uses thereof. The compositions include models for the study of developmental biology of the liver and other epithelial tissues, drug discovery and toxicity screening, infectious disease biology of various infectious agents, and personalized medicines. Methods for seeding canine intestinal organoids, maintaining an organoid monolayer, and monitoring monolayer integrity are provided. Methods and systems for culturing, freezing, and recovering the frozen organoid cells are also provided. The hepatic organoids may also be used to treat a subject in need, or for identifying a preferred therapeutic agent.

In vitro methods for generating induced pluripotent cells (iPSCs) are disclosed herein. Also encompassed are recombinant iPSCs generated using these methods and methods of use thereof.

A method of preparing pancreatic islet-like cell structures having a unique combination of high viability, morphological and functional features that make the pancreatic islet-like cell structures particularly suitable for use in both clinical and drug screening application is provided. Pancreatic islet-like cell structures obtained from the method are also provided.

The present invention provides a human cell population that can self-renew extensively and yet retain the capacity to differentiate into red blood cells (RBCs). These cells are referred to as extensively self-renewing erythroblasts (ESREs). The cells of the invention serve among other things as a renewable source of transfusable RBCs.

The present invention provides a human cell population that can self-renew extensively and yet retain the capacity to differentiate into red blood cells (RBCs). These cells are referred to as extensively self-renewing erythroblasts (ESREs). The cells of the invention serve among other things as a renewable source of transfusable RBCs.

The present invention relates to a method for preparing induced hepatic progenitor cells (iHPC) comprising the steps of dedifferentiation of hepatocytes by culture with a culture medium comprising at least one activator of the Wnt signaling, Basic fibroblast growth factor (b-FGF), and Epidermal growth factor (EGF).