The present disclosure provides cell-based compositions for treating diabetes, methods for identifying cells that preferentially differentiate into endoderm cells, and methods for preparing insulin-producing pancreatic cells, as well as related methods of use for treating diseases related to insulin deficiency.

The present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells of a mature phenotype. The resulting pancreatic endocrine cells express single hormonal insulin, PDX1, NKX6.1, and MAFA. In one or more differentiation stages, culturing may be carried out in a culture vessel at the air-liquid interface.

The present invention relates to methods of producing pancreatic endocrine cells and uses of the cells obtained using the methods. The method utilises inhibitors or combinations of factors to provide increased quantities of endocrine material, for example for transplantation purposes.

Disclosed herein are methods, compositions, kits, and agents useful for inducing cell maturation, and isolated populations of SC- cells for use in various applications, such as cell therapy.

Disclosed herein are methods, compositions, kits, and agents useful for inducing cell maturation, and isolated populations of SC- cells for use in various applications, such as cell therapy.

The present application discloses cell clusters resembling the function and characteristics of endogenous pancreatic islets, and methods for making and using such cell clusters.

A method for obtaining epithelial organoids is provided. In one embodiment, the method comprises culturing one or more epithelial ducts, epithelial duct fragments and/or epithelial stem cells isolated therefrom in contact with an extracellular matrix in the presence of a basal medium, wherein the medium is free of FGF and/or nicotinamide. Organoids obtained by the methods described herein, and uses thereof, are also provided.

Methods of identifying compounds that increase cell number and/or proliferation by determining the effect of compounds on cell number or proliferation in zebrafish pancreas are provided.

Disclosed herein are methods, compositions, kits, and agents useful for inducing cell maturation, and isolated populations of SC- cells for use in various applications, such as cell therapy.

Disclosed herein are methods for manufacturing transdifferentiated populations of non-pancreatic human insulin producing cells, and methods for enriching populations of non-pancreatic -cells for cells comprising an enriched capacity for transcription factor-induced transdifferentiation into a pancreatic -cell phenotype and function.