The present invention relates to a cell reprogramming method using a physical stimulation-mediated environmental influx, and more specifically, by subjecting differentiated or non-differentiated cells to physical stimulation which can promote an environmental influx, such as ultrasonic waves, laser or heat shock, without the introduction of a reprogramming-inducing factor or a chemical substance to the differentiated cells, the cells can be reprogrammed with just the imposition of an external environmental influx into pluripotent cells or arbitrary differentiated cells having a different expression type from the differentiated or non-differentiated cells, and as such an inducement has a simple and effective production process, the possibility of an autogenic cell therapy can be made greater.

In one embodiment, the present invention relates to a non-enzymatic method for isolating stem cells from adipose tissue, wherein the method comprises treating adipose tissue with ultrasonic cavitation to break up the adipose tissue and lyses mature adipocytes, resulting in a stromal vascular fraction containing viable stromal/stem cells.

Compositions and methods are provided for the generation of highly potent conditioned stem (Epinul) cells from adult somatic cells or tissues. Such conditioned stem cells are capable of generating all the cell lineages of any tissue or organ. Uses and compositions of the conditioned stem cells are also disclosed.

A method of forming an aggregate of objects in a channel including a liquid, the method including: a) providing objects in at least a region of the channel, and b) forming an aggregate of the objects by submitting them to a modulated pulsed acoustic field, wherein the modulated pulsed acoustic field applied at step b) is modulated in amplitude.

Disclosed herein is a system comprising acoustically assembled patient-derived cell clusters that can preserve original tissue compositions, including tumor and immune cell compositions for solid tumors collected from a patient, and model their interactions in 3D microenvironments. Such a system allows for ex vivo manipulations of the system to investigate and predict how the native tissues will responses to treatments, including those of a primary and metastatic patient tumor, in a rapid, scalable, and user-friendly manner.

The present invention is directed to methods of inducing spatial organization of cells an in vitro culture system using ultrasound technology. The invention is further directed to methods of inducing extracellular matrix remodeling and neovessel formation in an in vitro culture system and generating vascularized engineered tissue constructs using ultrasound technology.

The present invention is directed to methods of inducing spatial organization of cells an in vitro culture system using ultrasound technology. The invention is further directed to methods of inducing extracellular matrix remodeling and neovessel formation in an in vitro culture system and generating vascularized engineered tissue constructs using ultrasound technology.

An apparatus for use in promoting motility of flagellar cells includes an acoustic energy emission module configured to generate ultrasound energy, the module being configured to generate ultrasound waves within a frequency range of about 2 MHz and about 120 MHz, and an applicator module configured to direct the generated ultrasound waves to a locus of the cells for a duration of between about 5 seconds and about 35 seconds.

A method for elongating telomeres of cells comprises steps of: providing physical stimulation directly or indirectly to cells; and culturing a mixture of the cells and a medium for a predetermined time, wherein providing the stimulation directly to the cells comprises applying physical stimulation to the medium containing the cells, and providing the stimulation indirectly to the cells comprises applying physical stimulation to the medium not containing the cells and then mixing the medium and the cells. The method for elongating telomeres of cells is simpler than a conventional method and is superior in terms of time, cost, efficiency, and safety. In addition, the method induces cell division and provides an anti-aging effect, in addition to simply elongating telomeres. Thereby, it is expected that the method can ameliorate and prevent not only problems caused by shortening of telomeres, but also various aging-related diseases and conditions.

Disclosed herein include methods, compositions, and kits suitable for use in sorting a population of cells. In some embodiments, the method comprises flowing a fluid sample comprising a population of cells through a microfluidic channel. The population of cells can be configured to express gas vesicles (GVs) in a context-dependent manner. The expression of GVs within a cell can increase the compressibility () and reduce the density () of said cell, thereby modulating the acoustic contrast () of said cell relative to the fluid in the microfluidic channel. The method can comprise applying ultrasound to the microfluidic channel. Applying ultrasound can generate acoustic standing wave(s) in the microfluidic channel, thereby positioning pressure antinode(s) in the microfluidic channel.