The invention relates to a method for preparing an irradiated platelet lysate, comprising the steps of providing a starting platelet lysate having platelet factors including growth factors and plasma proteins including coagulation factors and proteins other than the coagulation factors. Double irradiation of the starting platelet lysate, using UVC radiation and ionizing radiation. The double irradiation with UVC radiation and ionizing radiation being arranged to retain at least 75% of the total protein concentration of the starting platelet lysate, while reducing, by at least 40%, the concentration of at least one of the coagulation factors including fibrinogen, factor II, factor VII, factor IX, factor X and factor XI of the starting platelet lysate.

A cell culture container is provided which is filled with a cell culture substrate (14) including a gel layer formed of a gel (15) capable of being denatured by heating and a gold nanoparticle layer (13) formed on one surface of the gel layer, in which a cell is cultured on a side of the one surface or a side of the other surface in the cell culture substrate. A method for manufacturing a cell culture container, including: a step of filling the cell culture container with a gel capable of being denatured by heating; and a step of forming a gold nanoparticle layer on one surface of the gel. A method for acquiring a cell, including: a step of selecting a cell to be acquired from cells placed in the cell culture container; a step of irradiating a cell culture substrate in the vicinity of the selected cell with light; and a step of recovering the selected cell.

A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.

Provided is a linker for both screening assessment of the candidate clones without using enzymes, and to provide an in vitro selection method using thereof. Also, provided is a high-speed photo-crosslinking linker for molecular interaction analysis and in vitro selection comprising a backbone and a side chain. The backbone comprises a solid-phase binding site located at the 5 terminus for forming a bond with a solid-phase; a solid-phase cleavage site for releasing the entire solid-phase at the site; a side chain linking site for linking a side chain; a high-speed photo-crosslinking site for linking the backbone to mRNA having a sequence complementary thereof via photo-crosslinking; and a reverse transcription initiation region located adjacent to the side chain linking site at the 3 terminus of the backbone. The side chain comprises a fluorescent label, a protein binding site located at the free terminus thereof; and a binding site with the backbone.

The present invention relates to products and methods for modulating cardiac function.

An object of the present invention is to obtain a thicker animal cell composition by a simple and less expensive method. Namely, an object of the present invention is to provide a method for culturing a thicker animal cell composition by eliminating the hypoxia associated with animal cell compositions, a method for producing an animal cell composition containing unicellular algae, and an animal cell composition. The present invention provides a method for culturing an animal cell composition in a culture medium in the presence of unicellular algae and under exposure to light. According to the method of the present invention, oxygen can be continuously supplied in the culture medium, cell damage is alleviated, and a thicker cell composition can be obtained in the absence of a capillary network.

A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.

The present disclosure provides a conjugate comprising: a) an affinity agent that specifically binds a target ligand-binding polypeptide; and b) a photoisomerizable regulator comprising: i) a photoisomerizable moiety; and ii) a ligand that binds to the target ligand-binding polypeptide. The present disclosure provides cells comprising a conjugate of the present disclosure. The present disclosure provides methods of using a conjugate of the present disclosure to modulate activity of a target polypeptide, and to modulate activity of a target cell or cell population.

A microsurgical probe employs an optional probe support structure; an optical fiber for providing a feed path for an emission wavelength; a chemical feed path for delivering a chemical; a resonator motor; and a probe accessory tool. A microsurgical system additionally employs a sensor and an artificial intelligence (AI) system to assess conditions based on data provided by the sensor. The system can be employed to remove tumor tissue that is interwoven with healthy tissue. This system can also be employed to fertilize old, inflexible ova.

A method for treating spermatozoa of an animal includes: irradiating the spermatozoa with light having wavelengths in a range of 390-420 nm at a fluence effective to increase motility of the spermatozoa by 75% or more.