Provided is a resin film formed of a cell culture scaffold material, which has excellent fixation of cells after seeding and is capable of enhancing proliferation rate of cells. A resin film formed of a cell culture scaffold material, in which the cell culture scaffold material contains a synthetic resin, and the resin film has phase-separated structure including least a first phase and a second phase, and a ratio of the surface area of one of the first phase and the second phase to the entire surface is 0.01 or more and 0.95 or less.

There is described a method of promoting the attachment, survival and/or proliferation of a stem cell in culture, the method comprising culturing a stem cell on a positively-charged support surface. There are also provided a cell composition prepared according to the method of the invention.

Disclosed herein is a cell culture surface, comprising: a polymer material comprising an acrylate functional group; and one or more functional compounds comprising one or more functional moieties, at least one moiety from the one or more functional moieties configured to adhere to animal cells; wherein the cell culture surface has a compressive modulus between 10 kPa to 1000 kPa. Also disclosed herein are systems and methods for making and using the same.

The present disclosure discloses a rosin-based small molecular weight hydrogelator and an application thereof, and belongs to the fields of supramolecular chemistry, surfactant science and chemical utilization of rosin. The rosin-based small molecular hydrogel of the present disclosure can gel water at a very low concentration, and the critical gelling concentration is only 0.176 wt %. On average, each gelling agent molecule can hold 13,889 water molecules, which exhibits extremely high gel efficiency and the formed small molecular hydrogel also exhibits extremely high stability. This small molecule hydrogel is derived from the natural product rosin and has a mild nature. It can be used in the fields of drug sustained-release, tissue engineering, daily chemicals, medicine and so on. At the same time, the rosin-based small molecular hydrogel 6-dehydroabietylamide amine oxide in the present disclosure can form a stable gel emulsion for most oils, and can be used in many fields such as food, medicine, daily chemicals, tissue engineering, environmental protection, and water pollution control.

The invention relates to a process for producing carrier particles for the cultivation of biological cells, comprising the steps of providing an aqueous suspension of hydrogel beads and freeze-drying of the hydrogel beads so that dry hydrogel particles are formed, wherein at least one lyoprotectant substance is added to the suspension, the hydrogel beads are loaded in the suspension with the lyoprotectant substance and the dry hydrogel particles receive a shape under the effect of the lyoprotectant substance, which shape approximates a spherical particle shape and is still maintained after rehydrating. The invention also relates to carrier particles for a cultivation of biological cells, which comprise dry hydrogel particles, preferably having a protein coating, and to applications of the carrier particles.

Systems and methods for creating cell sheets with high extracellular (ECM) content, while controlling cell alignment, are described. The method is simple, easy to perform, has a low-cost, and uses non-toxic and food-grade and food-safe materials. The method allows for cell alignment in sophisticated patterns using simple molding process with 3D printed molds prepared with cheap open-source 3D printers and using different types of filament materials. The method allows for the reuse of the silicone-based membranes by simple autoclaving and/or an isopropanol washing step. The method also creates multi-layer cell constructs and induced ECM production with optional ECM crosslinking using food-grade materials resulting in strong sheets formed in a short process that can be formed using simple scraping.

Provided herein are methods that relate, in some aspects, to the incubation or culturing, such as to induce stimulation of expansion (proliferation), activation, costimulation and/or survival, of a composition of cells, such as a population of lymphocytes. In some aspects, provided are methods and reagents for the stimulation, e.g., of expansion (proliferation), survival or persistence, activation, costimulation, or other effect, of cell populations that involve binding of agents to a molecule on the surface of the cells, thereby providing one or more signals to the cells. In some cases, the reagents are reagents containing a plurality of binding sites for agents, such as multimerization reagents, and thus the one or more agents are multimerized by reversibly binding to the reagent, e.g., thereby creating a stimulatory reagent (multimerized agent), having stimulatory agents multimerized thereon. In some aspects, the multimerized agent can provide for expansion or proliferation or other stimulation of a population of cells, and then such stimulatory agents can be removed by disruption of the reversible bond. Also provided are compositions, apparatus and methods of use thereof.

The present invention provides liquid crystals and compositions thereof (e.g., liquid crystal-based scaffolds). The present invention also provides the methods for generating said liquid crystals and compositions thereof (e.g., liquid crystal-based scaffolds) as well as uses thereof in cell-culturing and tissue-generating.

Embodiments herein described provide antigen-presenting cell-mimetic scaffolds (APC-MS) and use of such scaffolds to manipulating T-cells. More specifically, the scaffolds are useful for promoting growth, division, differentiation, expansion, proliferation, activity, viability, exhaustion, anergy, quiescence, apoptosis, or death of T-cells in various settings, e.g., in vitro, ex vivo, or in vivo. Embodiments described herein further relate to pharmaceutical compositions, kits, and packages containing such scaffolds. Additional embodiments relate to methods for making the scaffolds, compositions, and kits/packages. Also described herein are methods for using the scaffolds, compositions, and/or kits in the diagnosis or therapy of diseases such as cancers, immunodeficiency disorders, and/or autoimmune disorders.

Provided is a cell culture scaffold material having excellent extensibility of pseudopodia and excellent cell proliferation. The cell culture scaffold material according to the present invention contains a peptide-conjugated polyvinyl alcohol derivative having a polyvinyl alcohol derivative portion and a peptide portion, and has a hydroxyl value of 1,100 mgKOH/g or less.