This invention relates to live cell constructs for in vitro and/or ex vivo production of cultured milk products from mammary cells, methods of producing isolated cultured milk products from mammary cells, bioreactors for producing isolated cultured milk products, and cultured milk products.

The present invention relates to microcapsules comprising natural oil and, more specifically, to microcapsules which contain gelatin, natural polymers, oil, and an oil thickener and have enhanced mechanical properties, and a preparation method therefor. The microcapsules comprising natural oil according to the present invention have remarkably enhanced mechanical properties and retention properties, and when co-cultured with cells, the microcapsules provide the effect of inducing maturation of the cells, and thus may be used in various fields of microcarriers, cell cultures, and co-culture systems.

There is described a method of promoting the attachment, survival and/or proliferation of a stem cell in culture, the method comprising culturing a stem cell on a positively-charged support surface. There are also provided a cell composition prepared according to the method of the invention.

Disclosed herein is a cell culture surface, comprising: a polymer material comprising an acrylate functional group; and one or more functional compounds comprising one or more functional moieties, at least one moiety from the one or more functional moieties configured to adhere to animal cells; wherein the cell culture surface has a compressive modulus between 10 kPa to 1000 kPa. Also disclosed herein are systems and methods for making and using the same.

A cell-based device and method are disclosed for in vitro testing of compositions or compounds for irritation potential. Both the device and method comprise a) a confluent layer of corneal epithelial cells supported on a chemically patterned hydrogel, where the cells are aligned with the chemical pattern, b) a collector to receive effluent fluid, and optionally c) a detector. The device and method can also comprise an extracellular matrix (ECM) assembled by corneal keratocytes supported on the chemically patterned hydrogel. The cell-based device and method provide simple replacements for the in vivo Draize rabbit eye irritation test.

A cell culture substrate for cultivating adherent cells, including: a substrate (S), a polymer (P) comprising amino groups, which is bonded to the substrate, and a saccharide (Z) having at least two monosaccharide units for attaching the adherent cells, wherein the saccharide (Z) is covalently bonded to the polymer (P) via the amino groups.

Such a cell culture substrate is suitable for cultivating adherent cells and allows the cells to be detached from the cell culture substrate in a gentle manner by adding a saccharide.

Embodiments of the invention describe a cultured meat or food product with more desirable flavor and texture, as well as methods and compositions for producing the same. Certain embodiments are directed to methods of producing a stiffer, structured surfaces or scaffolds that mimic the extracellular matrix (ECM) and support the growth of myotubes that are interspersed with a scaffold component supporting fat cells (adipocytes) in vitro.

A cell culture article, including: a substrate comprising a polygalacturonic acid compound selected from at least one of: pectic acid; partially esterified pectic acid having a degree of esterification from 1 to 40 mol %, or salts thereof; and an adhesion polymer on the surface of the polygalacturonic acid compound. A method of making and using the article are also disclosed.

Gellan gum-based hydrogels are disclosed herein for in vitro cell culture and tissue engineering and regenerative medicine applications. Such gellan gum-based hydrogels may be used alone or combined with live cells and/or biomolecules for application in humans and/or animals. Chemical modification of gellan gum with selected ion-chelating substituents affords novel gellan gum hydrogels endowed with tunable physicochemical and biological properties. The modified gellan gum hydrogels described herein present advantages over existing hydrogel systems, including solubility, ionic crosslinking versatility, ease of formulation and injectability and greater adhesiveness within biological tissues and surfaces, whilst maintaining encapsulated cells viable during long culture periods and up-regulating the expression of healthy extracellular matrix markers.

Degradable hollow shell particles are disclosed that can encapsulate cells within the hollow inner cavity that allows for the high-throughput screening and sorting of the encapsulated cells based on their phenotypic properties. The solid-phase of the particle is porous such that solution exchange can occur between the external environment and the interior cavity. Further, the solid-phase contains degradable crosslinkers and can be degraded to release enclosed biological entities. An example embodiment consists of encapsulating a cell within the hollow shell particle, allowing the cell to accumulate biomass, selecting hollow shell particles based on accumulated biomass, and degrading the hollow shell particles to release the cells and develop hyper-producing cell lines. Exemplary cell types include microalgae, mammalian cells, bacteria, yeast, and fungi.