A diode and logic gate comprising cells is disclosed. A method of making the diode and logic gate comprising cells is disclosed.

Interlocking porous hydrogel blocks (IPHB), which are suitable for variety of cell and/or tissue growth, are provided. The IPHBs have a three-dimensional (3D) macrostructure defined by a continuous polymeric matrix material and a network of microporous channels and/or chambers extending throughout the continuous polymeric matrix material. The 3D macrostructure comprises a top surface, a bottom surface, and a thickness defined by at least one side edge extending from the top surface to the bottom surface. The 3D macrostructure structure includes at least one interlocking-male component and at least one interlocking-female component.

The present disclosure concerns a cell or tissue culture system comprising a solid support for the culture of adherent cells or adherent tissues and a plurality of cationic dendrimers associated to the surface of the solid support. Each cationic dendrimer includes one or more functional amine group. The cationic dendrimer is protonated at physiological pH. The cell or tissue culture system can be used for the culture of adherent cells or tissues and be used for the differentiation of stem cells.

A method of manufacturing a patterned substrate for culturing cells. The method includes the steps of: (1) preparing a substrate, (2) forming a first plasma polymer layer by integrating a first precursor material on the substrate using a plasma, wherein the first plasma layer inhibits cell adsorption, and wherein the first precursor material is a siloxane-based compound having a siloxane functional group with the SiOSi linkage, (3) placing a shadow mask having a predetermined pattern on the first plasma polymer layer thus formed, and (4) forming a second patterned plasma polymer layer by integrating a second precursor material using a plasma, wherein the second patterned plasma layer permits culturing of cells, whereby the patterned substrate is obtained.

A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

The present invention provides systems for cell separation based on cell rolling on surfaces along edges of regions coated with cell adhesion molecules. A variety of designs of coated regions and edges are disclosed.

A carrier for expansion of induced pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing induced pluripotent stem cells using the same carrier are also provided.

Compositions and methods are provided relating to in vitro differentiation of vascularized two-and three-dimensional biological structures, which may be referred to herein as vascularized organoids (VOs). Vascularization in the organoids is spatially organized, and can include hierarchical branching of large vessels to small vessels. Features of the vascularized organoids include vascular tissues having the same branching structure and self-organization as seen in vivo; and physiologically relevant spatial organization within endocardial, myocardial, epicardial, and/or progenitor cells. The organoids also have inherent vascular beds that can be anastomosed immediately with host vasculature beds, thereby improving transplantation efficiency by rapid oxygenation from blood flowing through their pre-incorporated vascular beds.

A method of screening a composition for cardiotoxicity comprising contacting the composition with cardiomyocytes that have increased fatty acid oxidation and/or diminished glucose oxidation. The cardiomyocytes are preferably prepared by overexpression of COX7A1. The cardiomyocytes are preferably provided in a micropatterned co-culture to provide a mature functional hPSC-CM cardiotoxicity model.

The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.