A cell patterning material, a method of preparing the cell patterning material, a cell patterning method using the cell patterning material, and a biosensor including patterned cells obtained by using the cell patterning method are provided. According to the present disclosure, cells may be conveniently and efficiently patterned and the time for applying external stimulation for patterning may be controlled. In addition, the patterned cells may have an excellent proliferation rate and excellent differentiation efficiency, and may be re-patterned in a different direction, and High-throughput screening using the patterned cells is possible.

The present invention provides a cell culture sheet comprising a plurality of recesses each having an opening of 1000 μm or less in diameter, wherein each recess has an inner circumferential face and a bottom face, wherein the inner circumferential face has a non-cell adhesive surface, and wherein the bottom face has a cell-adhesive surface; and a method for producing a cell culture sheet, the method comprising laminating of a layer having a non-cell adhesive surface and a layer having a cell-adhesive surface, wherein the layer having a non-cell adhesive surface has a plurality of through-holes of 1000 μm or less in diameter. The cell culture sheet of the present invention can be prepared simply and assists efficient cell culture operation, and therefore can preferably be used in the field of cell-based pharmaceutical preparations, for example, spheroid-containing pharmaceutical preparations etc.

The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.

The current disclosure describes a method of fabricating a nn where n=1 to 100 micro well plate that has a transparent film bottom where the film has embossed on the surface micro structures for the facilitation of cell growth and differentiation in particular cardiomyocyte cells derived from human induced pluripotent stem cells. In one embodiment, the micro well plate has an array of 384 locations molded out of thermoplastic onto which a micro embossed film is laminated to the bottom. The micro embossed features are fabricated such that that when the film is laminated to the plate, e.g., with adhesive or via welding, the embossed microstructures are located within the individual microplate well locations.

Embodiments of the presently disclosed subject matter provide biomimetic cell culture substrates comprising highly tunable patterned polymer nanofiber matrices capable of modulating expression of critical self-renewal factors and markers of cell-cell interaction to maintain stemness of human mesenchymal stem cells in vitro. Embodiments of the presently-disclosed subject matter also provide scalable, highly repeatable methods of making biomimetic cell culture substrates by hot pressing thermoplastic polymer films into femtosecond laser-ablated nanopore molds to form patterned polymer nanofiber matrices on flat thermoplastic substrates.

The present disclosure relates to nanolithographical cell patterning. In some aspects, the present disclosure provides materials and methods for making an oriented array.

A diode and logic gate comprising cells is disclosed. A method of making the diode and logic gate comprising cells is disclosed.

A method for in vitro activation and/or expansion of immune cells is provided, including the steps of: a) providing magnetic particles having multi-protrusive surface modified with at least one type of immuno-inducing substance, in which each magnetic particle includes a copolymer core, a polymer layer, a magnetic substance layer, and a silicon-based layer from the inside to the outside; b) providing a cell solution including at least one type of immune cell in the cell solution; and c) bringing the magnetic particles in contact with the cell solution, in which the at least one type of immuno-inducing substance on the surface of the magnetic particle activates and/or expands the at least one type of immune cell in the cell solution.

Methods to form a surface coating and surface pattern, which are based on adsorption of hydrocarbon chains that can be used with imaging optics to visualize macrophage fusion and multinucleated giant cell formation with living specimens are described.

In some embodiments, the present invention provides tissue compositions including a first silk scaffold comprising a plurality of epithelial cells, a second silk scaffold comprising a plurality of stromal cells, and a plurality of neurons. In some embodiments, provided compositions can function as physiologically relevant corneal model systems for, inter alia, testing of therapeutics for corneal disease and/or injury and production of functional corneal tissue (e.g., for transplant, etc). The present invention also provides methods for making and using provided compositions.