The present invention provides devices and methods for delivering a population of cells or a therapeutic agent to a subject in need thereof.

The present disclosure discloses a preparation method and use of a crosslinked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca.sup.2+ are crosslinked through physical crosslinking to form a double-network hydrogel with a high mechanical strength, the hydrogel is coated with heparin and collagen through dip coating, such that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, extracted primary muscle stem cells are inoculated onto the hydrogel and cultured in a growth medium (79% of DMEM, 10% of FBS and 1% of double antibodies) for 24 h. The cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 d. The hydrogel can enhance the absorption to nutrient substances by the muscle stem cells and facilitate growth of the muscle stem cells. The double-network hydrogel has the potential to be a scaffold for growth of muscle stem cells for cultured meat from stem cells.

Described is a crosslinked hydrogel for muscle stem cell culture and a preparation method and use thereof. The preparation method includes: dissolving collagen to prepare a solution and adding alginate and heparan sulfate proteoglycan and uniformly mixing with the collagen solution; adding ε-PL and TGase into the solution, uniformly stirring, and putting a slurry into a mold for crosslinking to obtain the hydrogel. The hydrogel is prepared by linking the collagen, the polylysine, and the heparan sulfate proteoglycan using the TGase to form covalent crosslinking, and forming a compact three-dimensional “egg box” network structure through a physical electrostatic interaction between the polylysine and the alginate.

Disclosed herein is a composite scaffold material that includes a crosslinked polymer matrix formed from a non-mammalian collagen and a crosslinking agent and/or a crosslinked polymer matrix formed from a non-mammalian collagen that has undergone self-crosslinking, and a plurality of calcium phosphate particles distributed within the crosslinked polymer matrix, where the composite scaffold material is porous. Also disclosed herein are methods of manufacturing the composite scaffold material and uses thereof. Further disclosed herein is a method of obtaining collagen from a non-mammalian source.

Embodiments of the present disclosure generally relate to methods and compositions for controlling cellular expression. More specifically, embodiments described herein relate to hydrogel-encapsulated/dispersed cells, methods of forming hydrogel-encapsulated/dispersed cells, and methods of using hydrogel-encapsulated/dispersed cells for controlling production of, for example, secretomes. In an embodiment, a composition for controlling production of secretomes is provided. The composition includes, a hydrogel comprising, in polymerized form, one or more photoreactive monomers and a thiol linker, wherein at least one of the one or more photoreactive monomers comprises a methylene functional group; and one or more cells dispersed or encapsulated within the hydrogel.

A microcapsule which is used in tissue regeneration, which may be specifically directed to the damaged tissues, and which forms an extracellular matrix-like structure at a certain point and thus allows cell proliferation.

Biomaterials for tissue regeneration and engineering applications and methods of making and use thereof are described, as well as constructs and kits derived from the biomaterials. The biomaterials can be derived from extracellular matrix and functionalized to make them crosslinkable and amenable to tuning of their material properties.

The present disclosure discloses a crosslinked hydrogel for muscle stem cell culture and a preparation method and use thereof, and belongs to the technical field of biological food materials. The preparation method includes: dissolving collagen to prepare a solution and adding a certain amount of alginate and heparan sulfate proteoglycan for being uniformly mixed with the collagen solution; and adding ε-PL and TGase into the solution, uniformly stirring, and putting a slurry into a mold for crosslinking to obtain the hydrogel. The hydrogel is prepared by linking the collagen, the polylysine and the heparan sulfate proteoglycan using the TGase to form covalent crosslinking, and forming a compact three-dimensional “egg box” network structure through a physical electrostatic interaction between the polylysine and the alginate. The hydrogel can enhance the absorption to nutrient substances by the muscle stem cells and facilitate the growth of the muscle stem cells. The double-network crosslinked hydrogel has the potential to be a scaffold for the growth of muscle stem cells for cultured meat from stem cells.

Provided is a method for evaluating a sample, the method including using a mixture containing a sample including at least one member selected from the group consisting of cell support-derived components, a cell suspension containing cells, an evaluation sample obtained from a cell suspension, a sample containing a liquid and microcarriers for use in cell culture, and a sample containing a liquid obtained following treatment of microcarriers, together with at least one substance selected from the group consisting of an aromatic compound having at least one functional group selected from the group consisting of a hydroxyl group, an amino group, a nitro group and a carbonyl group, and a fluorescent dye.

A technology of 3D printing integration of hard materials and cells, a preparation of bone-repair functional module with osteogenic microenvironment, bone organoid method and the application of quick repair of bone defects are provided. A preparation method of biological microenvironmental factors as independent osteogenic factors is further provided. The present integrated 3D printing technology realizes 3D printing of cells and hard materials synchronously by adjusting the temperature, so as to build a real sense of biomimetic bone tissue, which can be customized according to the specific defects and clinical needs of patients. In the present bone-repair functional module, the cells have high survival rate and proliferation activity on the surface of hard materials, and realize osteogenic differentiation and mineralization; after implantation, it has the dual metabolic functions of bone formation and bone resorption, promoting vascular and neurogenesis, improving elastic modulus and reducing stress shielding.