By using a graft polymer comprising a dendritic polymer with a styrene skeleton and a hydrophilic polymer grafted to a terminal thereof, a temperature-responsive substrate for cell culture having a temperature-responsive surface for cell culture that allows cells to be cultured with high efficiency and which yet allows cultured cells to be exfoliated in a short period of time and with high efficiency by simply changing the temperature of the substrate surface can be prepared conveniently. If this temperature-responsive substrate for cell culture is used, cells obtained from a variety of tissues can be cultured with high efficiency. If this culture method is utilized, cultured cells can be exfoliated intact in a short amount of time with high efficiency. In addition, by using this graft polymer, a wide range of peptides and proteins can also be separated by simply changing the temperature of a chromatographic carrier. This allows for convenient separation procedure and improves the efficiency of separating operations. What is more, the stereoregularity of the dendritic polymer per se may be utilized to enable separation of solutes based on differences in their molecular structures.

In some embodiments, the present disclosure provides cell systems that include arrangements of cells maintained by various separation systems and/or support layers for culture and differentiation of cells as well as related methods of use and preparation.

A cell culture substrate including: a substrate; and a layer containing hydrophilic polymer with a layer thickness of 5 to 2,000 nm covering at least a part of a surface of the substrate, in which the hydrophilic polymer contain a phosphorylcholine group or a hydroxyl group, the cell culture substrate has regions (A) below and regions (B) below, and an unevenness height at a boundary between each of the regions (A) and each of the regions (B) is 1 to 500 nm. (A): Island-shaped region of 0.001 to 5 mm.sup.2 in area with cell adhesiveness and cell proliferation properties (B): Region which is adjacent to region (A) and has no cell adhesiveness or cell proliferation properties

The present invention relates to a cell culture support comprising a substrate and a polymeric blend layer bound to the substrate. The polymeric blend layer comprises at least one thermoresponsive polymer and at least one coupling agent. The coupling agent is a non-protein coupling agent that has functional thiol, ester, epoxy, or aldehyde groups. The cell culture support further includes cells supported by the polymeric blend layer, wherein the thermoresponsive polymer provides for temperature induced detachment of the cells and/or cell sheets.

Provided is a novel method of manufacturing a cell-nanoscale thin film composite in which the cell-nanoscale thin film composite can be peeled from a substrate at a controlled timing. The method of manufacturing a cell-nanoscale thin film composite comprises culturing a cell in a cell culture base material in which a nanoscale thin film is provided on an electrode substrate with a self-assembled monolayer interposed therebetween, and reductively desorbing the self-assembled monolayer from the electrode substrate by applying an electric potential to the electrode substrate at a desired timing, so that the cell-nanoscale thin film composite is released.

Method of enhancing the cellular functions characterized by the inclusion of at least the process, wherein the aqueous solution containing the cells in the low temperature sol state, in an aqueous solution that exhibits thermoreversible sol-gel transition of being a sol at low temperatures and gel at high temperatures containing at least a hydrogel-forming polymer, bringing the solution containing the to a high temperature gel and then culturing the cells under microgravity.

The present invention provides for methods of isolating a stem cell or cell derived therefrom from a mixture of cells, for example, a mixture of adherent cells in culture. Cell isolation is achieved by the application of selective detachment forces.

The present invention provides for methods of isolating a stem cell or cell derived therefrom from a mixture of cells, for example, a mixture of adherent cells in culture. Cell isolation is achieved by the application of selective detachment forces.

A cell seeding substrate comprises a base comprising a rotary surface and a photolysis layer formed on the rotary surface of the base. The photolysis layer comprises a plurality of photolysis groups, and each of the plurality of photolysis groups has a chemical structural formula of

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R.sub.1 and R.sub.3 each represents alkane group, R.sub.2 comprises alkane group or olefin group, R.sub.5, R.sub.6 and R.sub.7 each represents hydrogen group or alkane group. Each of the plurality of photolysis groups is bonded to the rotary surface by the amide group.

Provided herein are methods and systems for bio-printing of three-dimensional organs and organoids. Also provided herein are bio-printed three-dimensional organs and organoids for use in the generation and/or the assessment of immunological products and/or immune responses. Also provided herein are methods and system for bio-printing three-dimensional matrices.