Disrupting convergence of lytic granules produced by cytotoxic lymphocytes allows non-directional degranulation, which improves and broadens killing efficiency of the cytotoxic cells in pathogenic environments such as when used for cancer therapy. Accordingly, methods of inducing multidirectional degranulation by cytotoxic effector cells in a tumor microenvironment, methods of treating a tumor, and related therapeutic composition are described.

A sheet-shaped cell culture comprising skeletal myoblasts for treating liver dysfunction or improving liver function. A method for producing the sheet-shaped cell culture, including a step of seeding a cell population comprising skeletal myoblasts on a culture substrate, a step of forming a sheet of the cell population into a sheet to form a sheet-shaped cell culture, and a step of detaching the formed sheet-shaped cell culture from the culture substrate. A method for treating liver dysfunction, including a step of applying the sheet-shaped cell culture to a site exhibiting the liver dysfunction.

A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

The present invention aims to provide an artificial tissue that can efficiently reproduce myocardial tissue function and that can be used in an actual implantation and produced by culturing. The present invention relates to a graft material for treating myocardial disease, the graft material including a cell sheet containing adipocytes.

Provided is a cell culture substrate, in which polymer (B) having a lower critical solution temperature contained in the substrate is a copolymer of a monomer (a) that becomes a hydrophobic polymer in homopolymerization and a monomer (b, c or d) that becomes a hydrophilic polymer in homopolymerization, which is uncrosslinked, and the lower critical solution temperature of the obtained copolymer (B) can be controlled widely by the types and ratio of the two monomers, to easily detach the cultured cells from the culture substrate surface rapidly without using protein hydrolase and collect the cells without damage. This cell culture substrate contains a polymer (A) of a (meth)acrylic acid ester monomer (a), one or more types of inorganic materials (C) selected from a water-swellable clay mineral and silica, and a polymer (B) having a lower critical solution temperature and including a monomer (a) and a monomer (b, c or d).

A cell treatment agent containing alginate sulfate as an active ingredient, and a set reagent for activating suspended or dormant cells, which is a combination reagent of the cell treatment agent and an activator containing polyvalent cations are provided.

The present invention provides liquid crystals and compositions thereof (e.g., liquid crystal-based scaffolds) for manufacturing various organoids, such as tumor organoids, as well as spheroids and/or 3D cell aggregates.

A method for micro-tissue encapsulation of cells includes coating a tissue scaffold stamp with an extracellular matrix compound; depositing the tissue scaffold stamp onto a thermoresponsive substrate; seeding the tissue scaffold stamp with a cell culture; incubating the cell culture on the tissue scaffold stamp at a temperature that is specified, wherein the cell culture forms a cell patch that is attached to the extracellular matrix compound; removing the thermoresponsive substrate by lowering the temperature; removing the tissue scaffold stamp from the cell patch to form a micro-tissue structure by dissolving the tissue scaffold stamp in a solvent; folding the micro-tissue structure by suspending the micro-tissue in the solvent to enable the cell patch to fold the micro-tissue structure; collecting the folded micro-tissue structure from the solvent; and administering the folded micro-tissue structure to an organism.

This invention provides a dynamic hydrogel that may deform in response to heat, the dynamic hydrogel is composed of a hydrogel composition and is obtained by crosslink reaction. The hydrogel composition includes photo initiator, PNIPAM, GelMA and annealed graphene oxide. Based on the weight of the hydrogel composition being 100 wt %, the content of the annealed graphene oxide is between 0.03 wt % to 0.15 wt % and the XPS absorption intensity ratio of sp.sup.2-C and sp.sup.3-C (sp.sup.2-C/sp.sup.3-C) is greater than 1. Meanwhile, this invention also provides a pulmonary fibrosis bionic chip containing the dynamic hydrogel.

A three-layer structure is comprised of an extra-cellular matrix, a sheet-shaped cell culture, and a biodegradable gel layer (single layer). By virtue of this three-layer structure, breakage of the sheet-shaped cell culture due to high spray pressure is reduced. The fibrin gel may be formed on one surface of the sheet-shaped cell culture by spraying of a thrombin liquid. A production method may involve culturing cells on a temperature-responsive cell culture substrate, dripping fibrinogen onto a sheet-shaped cell culture having an extra-cellular matrix on a lower surface thereof, further spraying a thrombin liquid onto the sheet-shaped cell culture from an oblique direction, whereby the result is a three-layer structure having a fibrin gel layer only on one surface of the sheet-shaped cell culture and having an extra-cellular matrix layer on the opposite surface.