A three-layer structure is comprised of an extra-cellular matrix, a sheet-shaped cell culture, and a biodegradable gel layer (single layer). By virtue of this three-layer structure, breakage of the sheet-shaped cell culture due to high spray pressure is reduced. The fibrin gel may be formed on one surface of the sheet-shaped cell culture by spraying of a thrombin liquid. A production method may involve culturing cells on a temperature-responsive cell culture substrate, dripping fibrinogen onto a sheet-shaped cell culture having an extra-cellular matrix on a lower surface thereof, further spraying a thrombin liquid onto the sheet-shaped cell culture from an oblique direction, whereby the result is a three-layer structure having a fibrin gel layer only on one surface of the sheet-shaped cell culture and having an extra-cellular matrix layer on the opposite surface.

The present invention provides: a method for producing mixed cells comprising cardiomyocytes, endothelial cells and mural cells from induced pluripotent stem cells, the method comprising (a) a step of producing cardiomyocytes from induced pluripotent stem cells and (b) a step of culturing the cardiomyocytes in the presence of VEGF; and a therapeutic agent for heart diseases, comprising the mixed cells produced by the method.

Systems and methods of the present disclosure include a cell culture, a method for producing the cell culture, a kit for producing the cell culture, a method for treating inflammatory disease using the cell culture, and the like, for treating inflammatory disease. The cell culture containing cells can be derived from skeletal muscle.

The present disclosure aims to provide a manufacturing method of a cell structure. The manufacturing method comprises a preparation step of preparing, on a culturing surface of a cell culture container, a first coated region coated with a temperature-responsive polymer and/or a temperature-responsive polymer composition, and a plurality of second coated regions located at an edge of the first coated region and coated with a cell adhesive substance; and a seeding and culturing step of seeding cells in the first coated region and the second coated regions and culturing the cells to produce a cell structure.

Provided herein are compositions, devices, and systems comprising copolymers of an N-alkyl acrylamide residue and a polyester comprising citric acid, polyethylene glycol, and glycerol 1,3-diglycerolate diacrylate, and methods of use and manufacture thereof.

Disclosed herein is a 4D printed programmable culture substrate with the self-morphing ability to accommodate the change in morphology of stem cells during differentiation. The 4D printed culture substrate includes a shape memory polymer that is configured for transformation from a first topographical shape to a second topographical shape during a predetermined time period in response to a stimulus, such as temperature. The first topographical shape may include micro-wells and the second topographical shape may include microgrooves, which can accommodate the growth and differentiation of neural stem cells.

The present disclosure provides a substrate for cell culture. Systems comprising the substrate, and methods for using and manufacturing the substrate are also disclosed herein.

By using a graft polymer comprising a dendritic polymer with a styrene skeleton and a hydrophilic polymer grafted to a terminal thereof, a temperature-responsive substrate for cell culture having a temperature-responsive surface for cell culture that allows cells to be cultured with high efficiency and which yet allows cultured cells to be exfoliated in a short period of time and with high efficiency by simply changing the temperature of the substrate surface can be prepared conveniently. If this temperature-responsive substrate for cell culture is used, cells obtained from a variety of tissues can be cultured with high efficiency. If this culture method is utilized, cultured cells can be exfoliated intact in a short amount of time with high efficiency. In addition, by using this graft polymer, a wide range of peptides and proteins can also be separated by simply changing the temperature of a chromatographic carrier. This allows for convenient separation procedure and improves the efficiency of separating operations. What is more, the stereoregularity of the dendritic polymer per se may be utilized to enable separation of solutes based on differences in their molecular structures.

Provided herein are methods and systems for bio-printing of three-dimensional organs and organoids. Also provided herein are bio-printed three-dimensional organs and organoids for use in the generation and/or the assessment of immunological products and/or immune responses. Also provided herein are methods and system for bio-printing three-dimensional matrices.

A cell culture article, including: a substrate comprising a polygalacturonic acid compound selected from at least one of: pectic acid; partially esterified pectic acid having a degree of esterification from 1 to 40 mol %, or salts thereof; and an adhesion polymer on the surface of the polygalacturonic acid compound. A method of making and using the article are also disclosed.