Modified bacteriophage, uses thereof, and compositions containing the modified bacteriophage are described. The compositions are useful for human treatment and may treat various conditions, including bacterial infections.

Disclosed here are recombinant bacteriophages with tail fibers encoded by at least two subsets of genomic fragments from different bacteriophage having different host ranges.

Provided is a chemically modified phage display platform and method of use thereof. More specifically, the present disclosure provides a chemically modified phage display library that incorporates 2-acetylphenylboronic acid (APBA) moieties to elicit dynamic covalent binding to the bacterial cell surface. The APBA-modified phage display libraries described herein are applicable to a wide array of bacterial strains and/or mammalian cells, paving the way to facile diagnosis and development of strain-specific antibiotics, and/or peptide-antibiotic conjugates for effective and targeted treatment. Also provided are therapeutic peptides, and pharmaceutical compositions thereof, that are identified by screening the phage display library of the present disclosure, and method of use of such therapeutic peptides for effective and targeted treatment.

A method of eliminating, reducing or preventing bacterial contamination of a surface, the method comprising dispersing a bacteriophage containing composition in air in proximity to the surface. In some embodiments, the bacteriophage containing composition is a powder dispersed by pressurizing a load chamber containing the powder so that the powder is expelled through an outlet. Also, devices for performing the method and a powder used in the method.

Provided are Cas9 enzymes that have mutations that enhance their properties, relative to un-mutated Cas9. The altered Cas9 enzymes exhibit i) an increased rate of spacer acquisition, or ii) increased cleavage efficiency of targets with NAG PAMs, or a combination of i) and ii). The altered Cas9 enzymes comprise an amino acid substitution of 1473 and K500 in a Streptococcus pyogenes or similar Cas9 enzyme. Also provided are polynucleotides, including expression vectors that encode the Cas9 enzymes, cells that contain the polynucleotides, and methods of making and using such cells. The disclosure includes tagging, or labelling bacteria, and for enhancing phage acquired immunity in bacteria, such as those used in industrial processes, including the food and beverage industry, such as the dairy industry. The food products are also included.

Provided is a chemically modified phage display platform and method of use thereof. More specifically, the present disclosure provides a chemically modified phage display library that incorporates 2-acetylphenylboronic acid (APBA) moieties to elicit dynamic covalent binding to the bacterial cell surface. The APBA-modified phage display libraries described herein are applicable to a wide array of bacterial strains and/or mammalian cells, paving the way to facile diagnosis and development of strain-specific antibiotics, and/or peptide-antibiotic conjugates for effective and targeted treatment. Also provided are therapeutic peptides, and pharmaceutical compositions thereof, that are identified by screening the phage display library of the present disclosure, and method of use of such therapeutic peptides for effective and targeted treatment.

A method for modifying the genome of a lytic target phage, uses of the method and products thereof are described. Compositions comprising such phage are also described. The compositions may be formulated as a medicament, which are useful for human treatment and may treat various conditions, including bacterial infections.

Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.

The present invention provides a binding assay that employs an activatable dormant bacteriophage carrying a reporter gene to qualitatively or quantitatively detect the presence of a substance of interest in a sample. Also provided is a simple culture device that is designed for manufacture and use in areas of limited resources. The device is useful for cell culture in such environments with limited resources because cells grow in paper just as they do in a culture dish.

A phage dispensing system comprises a user interface, a phage treatment computing system, a phage laboratory, and a phage dispensing apparatus, including a dispensing kiosk. The user interface allows a treating physician to request testing of a patient sample by the phage laboratory to identify a phage mixture treatment. The phage computing system arranges shipping of the sample and provision of the identified phage mixture treatment to a dispensing apparatus located near the patient. The dispensing apparatus has a stock of phage vials which are used to prepare and dispense doses of the identified phage mixture treatment.