The invention relates to an NHEJ or MMEJ detection construct, to NHEJ or MMEJ detection assays comprising said construct and to a method of screening for an NHEJ or MMEJ modulator comprising said construct.

Recombinant cells expressing membrane transport proteins are provided, along with methods for their use in various applications. These applications include, without limitation, industrial biotechnology and the reproduction/emulation of biochemical pathways or components thereof (e.g. photosynthetic pathways or components thereof). The recombinant cells may be provided as a component of a transgenic organism (e.g. a transgenic plant).

The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR systems, components, and methods for targeted modification of nucleic acids. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.

The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR systems, components, and methods for targeted modification of nucleic acids. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.

The present application discloses a dicarboxylic acid transporter and its encoding gene dit gene for increasing oil yield of Mucor circinelloides, the dit gene is cloned from the high-yield M. circinelloides WJ11, and the dit gene is transformed into M. circinelloides deficient strain Mu402, the dit gene is integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mc-Dit. The total fatty acid content of the Mc-Dit strain increased by 33.76% and the intracellular lipid content may reach up to 17.67% of the dry biomass.

Disclosed herein are systems, methods and components for targeted gene editing. Certain embodiments relate to a Cas protein lacking catalytic activity fused to a transposase. Also disclosed are systems that involve a Cas-transposase fusion protein, gRNA sequences and at least one mini-transposon for directing transpositions at user-defined genetic loci. Implementations of the system may involve disruption of a target gene or insertion of a payload sequence into a target nucleic acid.

The present invention provides a method of manufacturing circular nucleic acid vectors containing a transgene comprising: (a) contacting a host system with a template, wherein the template comprises at least one flanking cleavage site(s), and (i) at least one phage origin of replication (ORI); (ii) at least one Terminal Repeat (TR), and; (iii) a promoter sequence operatively linked to a transgene; (b) incubating the host system for a time sufficient for replication to occur resulting in circular nucleic acid production; and (c) recovering the circular nucleic acid production, wherein the circular nucleic acid self-anneals.

The object of the present invention is to provide a protein production method capable of producing an active protein with high efficiency even at a low temperature, and a cell-free protein synthesis kit. A protein production method including producing a protein with a reaction solution of a cell-free protein synthesis system containing either one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein. A cell-free protein synthesis kit including one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein, and a reaction solution of a cell-free protein synthesis system.

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.