Provided herein are genetically modified host cells, compositions, and methods for improved production of artemisinic acid. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of amorpha-4,11-diene 12-monooxygenase. Also provided herein are methods for screening for variants of cytochrome p450 enzymes that have increased enzymatic activity relative to a parental control enzyme.

Systems, methods and computer-readable media are provided for designing experiments for organisms at a first scale to generate first-scale performance data used in predicting performance of the organisms at a second, larger scale. The design includes determining first-scale screening conditions based at least in part upon the contribution of second-scale conditions to performance parameters of an organism at the second scale. The first-scale screening conditions include one or more proxies for second-scale conditions that cannot be replicated at first scale. The design determines first-scale screening parameters based at least in part upon computer modeling of the metabolism of the organism at the second scale.

Methods and systems to control flexible gas fermentation platforms for improved conversion of CO.sub.2 into products is developed and particularly relates to a control process and system to control a ratio of feedstock gases and maximize the concentration of inert components in a bioreactor tail gas stream and or bioreactor headspace. Improved carbon utilization results though providing the most beneficial ratio of substrates to the bioreactor of the fermentation process.

A process for preparing phenylacetic acid is provided. The process essentially includes the steps of providing one or more yeast strains belonging to the genus Yarrowia and mutants thereof, providing a culture medium including phenylalanine, transforming phenylalanine into phenylacetic acid by fermentation of the one or more yeast strains in the culture medium, the phenylacetic acid being contained in a fermentation broth obtained by the fermentation, and isolating the phenylacetic acid from the fermentation broth.

A process for preparing phenylacetic acid is provided. The process essentially includes the steps of providing one or more yeast strains belonging to the genus Yarrowia and mutants thereof, providing a culture medium including phenylalanine, transforming phenylalanine into phenylacetic acid by fermentation of the one or more yeast strains in the culture medium, the phenylacetic acid being contained in a fermentation broth obtained by the fermentation, and isolating the phenylacetic acid from the fermentation broth.

Among other things, the present disclosure provides biosynthesis polypeptides, methods, and non-naturally occurring microbial organisms for preparing various compounds such as 1,5-pentanediol, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, and 2-keto carboxylic acids.

Among other things, the present disclosure provides biosynthesis polypeptides, methods, and non-naturally occurring microbial organisms for preparing various compounds such as 1,5-pentanediol, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, and 2-keto carboxylic acids.

The present disclosure identifies pathways, mechanisms, systems and methods to confer production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in engineered and/or evolved methylotrophs such that these organisms efficiently convert C1 compounds, such as formate, formic acid, formaldehyde or methanol, to organic carbon-based products of interest, and in particular the use of organisms for the commercial production of various carbon-based products of interest.

The present disclosure identifies pathways, mechanisms, systems and methods to confer production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in engineered and/or evolved methylotrophs such that these organisms efficiently convert C1 compounds, such as formate, formic acid, formaldehyde or methanol, to organic carbon-based products of interest, and in particular the use of organisms for the commercial production of various carbon-based products of interest.

An integrated process and system for employing low conversion rWGS to prepare a gas fermentation feed stream from a CO.sub.2 source and a hydrogen source in order to produce at least one gas fermentation product. Low conversion rWGS reactors may (1) employ a wider selection of inorganic catalysts then rWGS reactors requiring high temperature operation, (2) allow for use of an electric heater instead of a fired heater to preheat feed stream to the low conversion rWGS reactor, and (3) extend rWGS catalyst life by reducing the amount of water produced in the rWGS reaction.