The present invention belongs to the field of microbial technology, and specifically relates to a strain of Hericium erinaceus HT-3, with a deposit number of CCTCC No: M 2018567. The present invention also relates to a screening method for Hericium erinaceus HT-3. The screening method comprises the steps of purifying the strain of Hericium erinaceus from a tissue block, fermenting and culturing the strain, soak extracting ergothioneine from the mycelium cells in the fermentation broth, detecting the ergothioine content in the fermentation broth. The Hericium erinaceus strain screened according to the present invention has high ergothioneine yield, and the screening method is simple in process, easy to be operated, and low in production cost.

Process for enriching microalgal biomass, in particular of the genus Nannochloropsis, with different polyunsaturated fatty acids, primarily docosahexaenoic acid (22:6 n-3, DHA), using oils that are rich in these fatty acids. The process comprises the following steps: Preparing a solution or an emulsion of lipids+emulsifying agent (BSA), Preparing the biomass concentrate to obtain a final concentration of mg/ml or greater, Enriching the biomass concentrate by adding the lipids or the emulsion thereof to the microalgal concentrate, Allowing the mixture to rest under constant stirring for at least 24 hours under lighting conditions.

The process allows for the simultaneous enrichment of microalgal biomass of the genus Nannochloropsis with eicosapentaenoic acid (20:5 n-3) (EPA) and docosahexaenoic acid (22:6 n-3) (DHA), to obtain a minimum EPA:DHA weight ratio of 10:1, with an EPA content of at least 10% of the total fatty acid content.

Process for enriching microalgal biomass, in particular of the genus Nannochloropsis, with different polyunsaturated fatty acids, primarily docosahexaenoic acid (22:6 n-3, DHA), using oils that are rich in these fatty acids. The process comprises the following steps: Preparing a solution or an emulsion of lipids+emulsifying agent (BSA), Preparing the biomass concentrate to obtain a final concentration of mg/ml or greater, Enriching the biomass concentrate by adding the lipids or the emulsion thereof to the microalgal concentrate, Allowing the mixture to rest under constant stirring for at least 24 hours under lighting conditions.

The process allows for the simultaneous enrichment of microalgal biomass of the genus Nannochloropsis with eicosapentaenoic acid (20:5 n-3) (EPA) and docosahexaenoic acid (22:6 n-3) (DHA), to obtain a minimum EPA:DHA weight ratio of 10:1, with an EPA content of at least 10% of the total fatty acid content.

The present invention relates to recombinant bacteria producing L-amino acid, in which the recombinant bacteria has reduced expression of the glucose-6-phosphate isomerase gene pgi and improved expression of the glucose-6-phosphate dehydrogenase gene -opcA than the starting bacteria, where the starting bacterium is a bacterial strain that can accumulate target amino acid(s) and preferably, the amino acid is L-histidine.

The present invention provides methods for producing cannabinoid prodrugs. Also described are pharmaceuticals acceptable compositions of the prodrugs and a system for the large-scale production of the prodrugs.

The present invention provides methods for producing cannabinoid prodrugs. Also described are pharmaceuticals acceptable compositions of the prodrugs and a system for the large-scale production of the prodrugs.

A novel process for the preparation of L-threo-dihydroxyphenylserine (Droxidopa) is described. It comprises of enantioselective hydrolysis of racemic (DL)-threo-N-acetyl-3-(3,4-methylenedioxyphenyl)-serine using commercially available L-amino acylase from Aspergillus sp. (EC 3.5.1.14) in the presence of cobalt ions, to obtain (L)-threo-3-(3,4-methylenedioxyphenyl)-serine followed by dealkylation to obtain Droxidopa. Protecting the amino group of (L)-threo-3-(3,4-methylenedioxyphenyl)-serine using either benzyloxycarbonyl or phthaloyl group before dealkylation followed by deprotection of the amino group results in obtaining Droxidopa in high yields and purity.

Enzyme compositions with enhanced enzyme activity and/or thermophilic and psychrophilic stability are described. Additionally, methods and kits for making and using the enzyme compositions are provided.

Enzyme compositions with enhanced enzyme activity and/or thermophilic and psychrophilic stability are described. Additionally, methods and kits for making and using the enzyme compositions are provided.

Provided are a Corynebacterium glutamicum mutant that is resistant to high concentrations of L-glutamine, and a method of producing L-glutamine by using the mutant.