The present invention provides a method for bioconversion of mogroside extracts into siamenoside I, comprising: using (1) DbExg1 protein or (2) a microorganism expressing the DbExg1 protein to contact or to cultivate with the mogroside extracts. The present invention can convert the mogroside extracts into siamenoside I, which has a higher sweetening power and better taste than other mogrosides. The method of the present invention uses a microorganism expressing the responsible enzyme, DbExg1, which was identified as a mediator of mogroside V conversion into siamenoside I in the present invention, so that siamenoside I was preferentially produced. Thus, the use of the method of the present invention provides a feasible approach to produce large quantities of the natural sweetener, siamenoside I, which can then be applied in several industries.

The present invention provides a method for bioconversion of mogroside extracts into siamenoside I, comprising: using (1) DbExg1 protein or (2) a microorganism expressing the DbExg1 protein to contact or to cultivate with the mogroside extracts. The present invention can convert the mogroside extracts into siamenoside I, which has a higher sweetening power and better taste than other mogrosides. The method of the present invention uses a microorganism expressing the responsible enzyme, DbExg1, which was identified as a mediator of mogroside V conversion into siamenoside I in the present invention, so that siamenoside I was preferentially produced. Thus, the use of the method of the present invention provides a feasible approach to produce large quantities of the natural sweetener, siamenoside I, which can then be applied in several industries.

The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog. V), Mogroside VI (Mog. VI), Iso-Mogroside V (Isomog. V), and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog, V. Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.

Provided herein include methods of making mogroside compounds, e.g., Compound 1, compositions (for example host cells) for making the mogroside compounds, and the mogroside compounds made by the methods disclosed herein, and compositions (for example, cell lysates) and recombinant cells comprising the mogroside compounds (e.g., Compound 1). Also provided herein are novel cucurbitadienol synthases and the use thereof.

Provided herein include methods of making mogroside compounds, e.g., Compound 1, compositions (for example host cells) for making the mogroside compounds, and the mogroside compounds made by the methods disclosed herein, and compositions (for example, cell lysates) and recombinant cells comprising the mogroside compounds (e.g., Compound 1). Also provided herein are novel cucurbitadienol synthases and the use thereof.

Provided are a recombinant yeast having improved ability to produce ginsenoside, which is prepared by overexpressing INO2 and INO4 or deleting OPT1 in a yeast having ability to produce ginsenoside, a method of preparing the yeast, and a method of producing ginsenoside by using the yeast.

Provided are a recombinant yeast having improved ability to produce ginsenoside, which is prepared by overexpressing INO2 and INO4 or deleting OPT1 in a yeast having ability to produce ginsenoside, a method of preparing the yeast, and a method of producing ginsenoside by using the yeast.

The present invention relates to a method of producing ginsenoside 20(S)Rg3 or 20(S)Rh2 using a novel ginsenoside glycosidase in order to obtain ginsenoside 20(S)Rg3 or 20(S)Rh2 with high efficiency and high purity. According to the production method of the present invention, a large amount of 20(S)Rg3 or 20(S)Rh2, which is a minor form of rare ginsenoside present in very small amounts in ginseng or processed ginseng products, may be safely and efficiently produced. In particular, the method according to the present invention has an advantage in that it may produce a large amount of 20(S)Rg3 or 20(S)Rh2 for industrial applications, since the process is very simple and the production efficiency is very high.

The present invention relates to a method of producing ginsenoside 20(S)Rg3 or 20(S)Rh2 using a novel ginsenoside glycosidase in order to obtain ginsenoside 20(S)Rg3 or 20(S)Rh2 with high efficiency and high purity. According to the production method of the present invention, a large amount of 20(S)Rg3 or 20(S)Rh2, which is a minor form of rare ginsenoside present in very small amounts in ginseng or processed ginseng products, may be safely and efficiently produced. In particular, the method according to the present invention has an advantage in that it may produce a large amount of 20(S)Rg3 or 20(S)Rh2 for industrial applications, since the process is very simple and the production efficiency is very high.

The present disclosure provides use of a timosaponin enzymatically transformed product in preparation of drugs for inhibiting skin superficial fungi. In the use provided in the present disclosure, the timosaponin enzymatically transformed product is prepared by enzymatically transforming rhizoma anemarrhenae extract with -glucosidase or a compound enzyme of xylanase and cellulose after purification. In the present disclosure, as an active pharmaceutical ingredient for inhibiting skin superficial fungi, the timosaponin enzymatically transformed product has the minimum inhibitory concentration of 4 mg/L against fungi, which is obviously superior to total timosaponin in the bacteriostatic capability, and has a certain application prospect in the aspect of treating skin superficial mycoses.