Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.

Disclosed herein are methods and systems for rapid detection of microorganisms such as Cronobacter spp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Cronobacter-specific bacteriophage, allows detection of a specific microorganism, such as Cronobacter spp. and an indicator signal may be amplified to optimize assay sensitivity.

Various embodiments of a light detection device and a method of using the device are disclosed. In one or more embodiments, the light detection device can include a housing that extends along a housing axis between top and bottom surfaces. The device can also include a port that is adapted to receive a sample, and a door connected to the housing. The door can include an actuator portion adapted to selectively move the door between a closed position and an open position, and a cover portion connected to the actuator portion and adapted to close the port when the door is in the closed position and open the port when the door is in the open position to allow external access to the port.

A method for detecting lactic acid and/or acetic acid bacteria in a food-processing matrix comprising a microbial flora (the microbial flora comprising a lactic acid and/or acetic acid bacterial flora) and a fungal flora, the bacterial flora containing an adenosine triphosphate of bacterial origin, the fungal flora containing an adenosine triphosphate of fungal origin, which comprises the following steps: applying, to the matrix, an antifungal having an antifungal action which is lethal, at a first time limit, on the fungal flora, and an antibiotic action which is non lethal, at a second time limit after the first time limit, on the bacterial flora, detecting the microbial flora between the first time limit and the second time limit; in which the lethal antifungal action releases, into the matrix, for the first time limit, adenosine triphosphate of fungal origin and in which the microbial flora is detected between the first time limit and the second time limit by means of the following steps: removing the free adenosine triphosphate from the matrix, then applying a lysis agent to the matrix, in order to release from the matrix the adenosine triphosphate of bacterial origin, then measuring the adenosine triphosphate released.

Provided herein is a recombinant cell comprising a deletion of a gene encoding a transporter associated with antigen processing (TAP) protein and mutations in the CD3 epsilon gene and an HLA-A gene. Also provided are systems and methods for screening for alloreactivity and specificity of an immunotherapeutic agent, such as a bispecific T cell engager or an engineered T cell receptor (TCR).