Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.

A method of testing a liquid sample for the presence of an analyte, the method comprising the steps of: forming a mixture by contacting the sample with a composition comprising an oxidase for formation of hydrogen peroxide from the analyte, a fluorescent indicator precursor capable of forming a fluorescent indicator in the presence of an oxygen radical and an iron compound wherein the iron compound is dissolved in the mixture; irradiating the mixture; and measuring fluorescence from the fluorescent indicator. The method may be carried out using a device in which the mixture in a channel or chamber (101) of a microfluidic device is irradiated by light from light source (103) and emission from the fluorescent indicator is detected by photodetector (105).

The present disclosure provides methods to identify peptides and small molecule moieties that are able to modulate protein-protein interactions (PPIs). Some moieties can disrupt specific PPIs within a complex, or disrupt variant-specific PPIs. Some moieties can alternatively bridge between two proteins in a protein-specific or a variant-specific manner. The methods described enable generation of compounds able to modulate PPI networks within cells with implications for drug development for pathological conditions.

Embodiments of the present disclosure relates to various bisulfite-free chemical methods for detecting methylation of cytosine in the DNA sample. These methods convert methylated and hydroxymethylated cytosine in the nucleic acid sequence to a modified or pseudo thymine or a uracil moiety which then can be detected in sequencing.

Embodiments of the present disclosure relates to various bisulfite-free chemical methods for detecting methylation of cytosine in the DNA sample. These methods convert methylated and hydroxymethylated cytosine in the nucleic acid sequence to a modified or pseudo thymine or a uracil moiety which then can be detected in sequencing.

The present disclosure provides compositions and methods related to TET-assisted oxidation of methylated nucleotide bases. In particular, the present disclosure provides optimized oxidation methods which provide more efficient and complete oxidation. The disclosed methods may be used to identify and detect the methylated nucleotide bases, particularly in conjunction with known TET-assisted sequencing methods.

The present disclosure provides compositions and methods related to TET-assisted oxidation of methylated nucleotide bases. In particular, the present disclosure provides optimized oxidation methods which provide more efficient and complete oxidation. The disclosed methods may be used to identify and detect the methylated nucleotide bases, particularly in conjunction with known TET-assisted sequencing methods.

The present disclosure provides methods to identify peptides and small molecule moieties that are able to modulate protein-protein interactions (PPIs). Some moieties can disrupt specific PPIs within a complex, or disrupt variant-specific PPIs. Some moieties can alternatively bridge between two proteins in a protein-specific or a variant-specific manner. The methods described enable generation of compounds able to modulate PPI networks within cells with implications for drug development for pathological conditions.