The invention relates to a system (10) for the amplification of a nucleic acid (22), comprising at least one local heating element (12), which is functionalized with at least one connection nucleic acid (14), and at least one primer nucleic acid (16), which is adapted to bind to the at least one connection nucleic acid (14) and to bind to the nucleic acid (22), and/or at least one primer complementary nucleic acid (30), which is adapted to bind to the at least one connection nucleic acid (14) and to elongate the connection nucleic acid (14) by a primer nucleotide sequence by means of an enzymatic reaction. Furthermore, the invention relates to a primer nucleic acid (16), a primer complementary nucleic acid (30), a local heating element (12) and a method for the amplification of a nucleic acid (22).

Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences for detection of coronavirus, including multiplex lateral flow diagnostic devices and methods of use, are provided.

Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences for detection of coronavirus, including multiplex lateral flow diagnostic devices and methods of use, are provided.

The present invention relates to methods of identifying viral hemorrhagic septicemia virus (VHSV) and detecting mutations in VHSV by use of a PNA probe that binds specifically to VHSV non-virion gene and by analysis of the melting pattern of the PNA probe. According to the present invention, a mutation in a virus that causes infectious disease in aquatic organisms can be detected in a simple, rapid and accurate manner, infection of fish with the virus can be detected, and a mutation in the NV gene can be detected.

The present invention relates to methods of identifying viral hemorrhagic septicemia virus (VHSV) and detecting mutations in VHSV by use of a PNA probe that binds specifically to VHSV non-virion gene and by analysis of the melting pattern of the PNA probe. According to the present invention, a mutation in a virus that causes infectious disease in aquatic organisms can be detected in a simple, rapid and accurate manner, infection of fish with the virus can be detected, and a mutation in the NV gene can be detected.

The present invention relates to a method for providing an analytical signal for determination of the presence of a target nucleic acid sequence in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention allows detection of a target nucleic acid sequence in a more accurate, effective and reproducible manner, by removing or adjusting a signal region that may affect the detection of a target nucleic acid sequence.

The present invention relates to a method for providing an analytical signal for determination of the presence of a target nucleic acid sequence in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention allows detection of a target nucleic acid sequence in a more accurate, effective and reproducible manner, by removing or adjusting a signal region that may affect the detection of a target nucleic acid sequence.

The present invention relates to probes and primers beneficial for conducting amplification assays, such as those including loop-mediated isothermal amplification reactions. Also described herein are methods for detecting targets using such probes and/or primers.

The present invention relates to probes and primers beneficial for conducting amplification assays, such as those including loop-mediated isothermal amplification reactions. Also described herein are methods for detecting targets using such probes and/or primers.

Described herein are systems and methods for multiplexed analysis of two or more targets in a test sample including a first set of particles including a first set of target-specific reagents and a first optically detectable identifier capable of emitting a first wavelength indicative of a first target, and at least one second set of particles including a second set of target-specific reagents and a second optically detectable identifier capable of emitting a second wavelength indicative of a second target; and at least one optically detectable reporter probe capable of constitutively emitting a third wavelength in response to reaction of the first set of target-specific reagents with the first target in the test sample and/or reaction of the second set of target-specific reagents with the second target in the test sample, wherein the first wavelength, the second wavelength, and the third wavelength are optically discernable from one another.