The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.

The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.

One aspect of the invention provides a method of separating long polynucleotides from a composition. The method includes: introducing a composition including one or more long polynucleotides into a container including at least one boundary defined by a filter comprising a plurality of pores, wherein the pores have a smaller cross-sectional dimension than specified for the long polynucleotide's molecular weight; and applying elevated hydraulic pressure to the composition, thereby causing at least some of the one or more long polynucleotides to pass through the pores.

One aspect of the invention provides a method of separating long polynucleotides from a composition. The method includes: introducing a composition including one or more long polynucleotides into a container including at least one boundary defined by a filter comprising a plurality of pores, wherein the pores have a smaller cross-sectional dimension than specified for the long polynucleotide's molecular weight; and applying elevated hydraulic pressure to the composition, thereby causing at least some of the one or more long polynucleotides to pass through the pores.

Provided herein are methods and systems for transfer target molecules to a surface, such as a planar surface. The transferred target molecules can be used for downstream applications, such as sequence identification.

Provided herein are methods and systems for transfer target molecules to a surface, such as a planar surface. The transferred target molecules can be used for downstream applications, such as sequence identification.

Instruments, methods, and kits are disclosed for performing fast thermocycling.

Instruments, methods, and kits are disclosed for performing fast thermocycling.

Described herein are bioaerosol detection systems and methods of use. In embodiments of bioaerosol detection systems and methods of use as described herein, systems and methods detect one or more coronaviruses and/or one or more influenza strains. In embodiments, coronaviruses according to systems and methods as described herein are SARS-CoV-2 coronavirus. In embodiments, influenza strains and/or particles according to the present disclosure are H1N1 influenza.

Described herein are bioaerosol detection systems and methods of use. In embodiments of bioaerosol detection systems and methods of use as described herein, systems and methods detect one or more coronaviruses and/or one or more influenza strains. In embodiments, coronaviruses according to systems and methods as described herein are SARS-CoV-2 coronavirus. In embodiments, influenza strains and/or particles according to the present disclosure are H1N1 influenza.