The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.

The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.

In a method for the desorption of nucleic acids from a sample, in order to simplify the desorption of nucleic acids from the sample, a solid phase is repeatedly rinsed with an elution buffer in a microfluidic system, in order to elute nucleic acids bonded to the solid phase from the solid phase in the microfluidic system.

In a method for the desorption of nucleic acids from a sample, in order to simplify the desorption of nucleic acids from the sample, a solid phase is repeatedly rinsed with an elution buffer in a microfluidic system, in order to elute nucleic acids bonded to the solid phase from the solid phase in the microfluidic system.

Provided herein, in some embodiments, are enzymatic methods for producing an oligonucleotide comprising phosphoramidate-linked nucleotides, and compositions comprising the oligonucleotide thus produced.

Provided herein, in some embodiments, are enzymatic methods for producing an oligonucleotide comprising phosphoramidate-linked nucleotides, and compositions comprising the oligonucleotide thus produced.

The invention provides compositions, devices, methods and kits allowing for rapid diagnosis of infectious diseases via extraction-free, direct PCR techniques using combined biological samples.

The invention provides compositions, devices, methods and kits allowing for rapid diagnosis of infectious diseases via extraction-free, direct PCR techniques using combined biological samples.

Provided herein is a reverse transcriptase mixture comprising a reverse transcriptase and a colored dye at a concentration in the range of 0.003%-1% (v/w). The colored dye may be visually observed during transfer of the mix from one vessel to another and addition of the mix to another mix can be confirmed by eye by observing the colored dye.

The present disclosure provides methods of preparing a fixed biological sample for use in an assay, wherein the method includes treatment of the sample with a low temperature active protease, optionally, in combination with an un-fixing reagent. The disclosure also provides assay methods, include partition-based methods, for fixed biological sample that use the low temperature protease treatment in combination with an un-fixing reagent. Kits comprising protease compositions, un-fixing agent compositions, and other assay reagents for use in the methods are also provided.