A kit and method for extracting nucleic acids from complex samples. The kit includes: (i) a lysis buffer having a concentration of SDS ranging from about 1% to about 25%; (ii) a buffer having a concentration of a potassium salt of ranging from about 0.1 M to about 5.0 M; (iii) a buffer having a concentration of a zinc and/or copper salt of ranging from about 0.5 M to about 5.0 M; (iv) a filter having a pore diameter ranging from about 1 μm to about 10 μm; and optionally, a member selected from one or more syringe(s), one or more reaction tube(s), an instruction guide, and any combination thereof.

A kit and method for extracting nucleic acids from complex samples. The kit includes: (i) a lysis buffer having a concentration of SDS ranging from about 1% to about 25%; (ii) a buffer having a concentration of a potassium salt of ranging from about 0.1 M to about 5.0 M; (iii) a buffer having a concentration of a zinc and/or copper salt of ranging from about 0.5 M to about 5.0 M; (iv) a filter having a pore diameter ranging from about 1 μm to about 10 μm; and optionally, a member selected from one or more syringe(s), one or more reaction tube(s), an instruction guide, and any combination thereof.

Provided herein, in some embodiments, are methods of purifying low-salt RNA compositions using denaturing oligo-dT chromatography.

Provided herein, in some embodiments, are methods of purifying low-salt RNA compositions using denaturing oligo-dT chromatography.

The present disclosure provides methods of reducing, minimizing, or inhibiting the formation of double-stranded ribonucleic acid (dsRNA) during the preparation of ribonucleic acid (RNA), such as messenger ribonucleic acid (mRNA), by adding at least one chaotropic agent to a starting reaction mixture. The present disclosure provides methods of reducing, minimizing, or inhibiting intramolecular base-pairing within a ribonucleic acid (RNA) transcript and/or intermolecular base-pairing between an RNA transcript and deoxyribonucleic acid (DNA) or another RNA during the preparation of ribonucleic acid (RNA), by adding at least one chaotropic agent to a starting reaction mixture.

The present disclosure provides methods of reducing, minimizing, or inhibiting the formation of double-stranded ribonucleic acid (dsRNA) during the preparation of ribonucleic acid (RNA), such as messenger ribonucleic acid (mRNA), by adding at least one chaotropic agent to a starting reaction mixture. The present disclosure provides methods of reducing, minimizing, or inhibiting intramolecular base-pairing within a ribonucleic acid (RNA) transcript and/or intermolecular base-pairing between an RNA transcript and deoxyribonucleic acid (DNA) or another RNA during the preparation of ribonucleic acid (RNA), by adding at least one chaotropic agent to a starting reaction mixture.

The invention, in some aspects, pertains to compositions and methods for selective extraction and purification of RNA.

The invention, in some aspects, pertains to compositions and methods for selective extraction and purification of RNA.

A transport medium is disclosed that can be utilized for both sample collection and molecular diagnostic applications. The transport medium can be utilized with multiple types of biological samples and maintains the stability of nucleic acid present in the biological samples so that one or more nucleic acid assay target(s) present in the biological sample is not substantially degraded during storage and shipping. Also disclosed are kits containing the transport medium, mixtures that include a biological sample disposed in the transport medium, and methods of producing and using the medium.

A transport medium is disclosed that can be utilized for both sample collection and molecular diagnostic applications. The transport medium can be utilized with multiple types of biological samples and maintains the stability of nucleic acid present in the biological samples so that one or more nucleic acid assay target(s) present in the biological sample is not substantially degraded during storage and shipping. Also disclosed are kits containing the transport medium, mixtures that include a biological sample disposed in the transport medium, and methods of producing and using the medium.