The present disclosure provides systems, devices, and methods that relate to a molecular recognition device configured to at least one of sense, identify and sequence at least one portion of a target molecule, the device comprising (a) a partition having a first side and a second side; (b) at least one constriction having a first end open to the first side and a second end open to the second side; (c) at least one pair of first and second sensing electrodes arranged within the constriction between the first side and the second side of the partition; and (d) a layer of a porous material at least one of arranged and formed on the first side of the partition.

The present disclosure provides systems, devices, and methods that relate to a molecular recognition device configured to at least one of sense, identify and sequence at least one portion of a target molecule, the device comprising (a) a partition having a first side and a second side; (b) at least one constriction having a first end open to the first side and a second end open to the second side; (c) at least one pair of first and second sensing electrodes arranged within the constriction between the first side and the second side of the partition; and (d) a layer of a porous material at least one of arranged and formed on the first side of the partition.

Barcoded ligation assay products from individual samples.

Barcoded ligation assay products from individual samples.

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

A preparation method for an aerolysin nanopore in this disclosure comprises the following steps: (1) pretreatment of an aerolysin; (2) preparation of a lipid bilayer membrane by pulling process; (3) forming of the aerolysin nanopore: the aerolysin nanopore is obtained at a current of 505 pA. The aerolysin nanopore prepared in the invention is structurally stable and has a high resolution with the whole internal cavity carried with a positive charge, can be used for detection without modification and is easily operated. Further, the aerolysin nanopore can be applied in DNA sequencing, DNA damage and Micro-RNA detection.

A preparation method for an aerolysin nanopore in this disclosure comprises the following steps: (1) pretreatment of an aerolysin; (2) preparation of a lipid bilayer membrane by pulling process; (3) forming of the aerolysin nanopore: the aerolysin nanopore is obtained at a current of 505 pA. The aerolysin nanopore prepared in the invention is structurally stable and has a high resolution with the whole internal cavity carried with a positive charge, can be used for detection without modification and is easily operated. Further, the aerolysin nanopore can be applied in DNA sequencing, DNA damage and Micro-RNA detection.

Provided herein are methods of determining a location of a biological analyte in a biological sample.

Provided herein are methods of determining a location of a biological analyte in a biological sample.