A method for seeding and amplifying target nucleic acids derived from a sample in a cluster at a site on a surface of a substrate includes retaining at least a portion of the target nucleic acids in an inactive form that cannot seed to provide a relatively low concentration of active form target nucleic acids available for seeding. As the active form target nucleic acids seed on the surface of the substrate, they may be amplified. Because the concentration of active form target nucleic acids is low, the likelihood is low that a second active form target nucleic acid will seed at the same site within the same cluster before the first active form target nucleic acid is sufficiently amplified to dominate. Accordingly, the likelihood that the cluster will pass filters is increased relative to traditional seeding and amplification methods employing a higher concentration of active form target nucleic acids.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

Methods for preparing sequencing libraries from a DNA-containing test sample, as well as methods for correcting sequencing-derived errors, are provided.

Methods for preparing sequencing libraries from a DNA-containing test sample, as well as methods for correcting sequencing-derived errors, are provided.

The present disclosure provides an anti-pollution consumable and a method for Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) molecular diagnosis using the same, belonging to the technical field of nucleic acid detection and molecular diagnostics. The anti-pollution consumable includes an outer reaction tube, a sleeve and an inner reaction tube, where the inner reaction tube includes a second hollow cylindrical upper body and a second-type conical lower body sequentially from top to bottom; a top end of the second hollow cylindrical upper body is externally connected with a fixing ring perpendicular to the second hollow cylindrical upper body; a number of drain holes are provided at a bottom of the second-type conical lower body; the drain hole has a diameter of 0.01-0.8 mm; the drain hole is used for hydrophobic treatment; and the inner reaction tube is fixed inside the outer reaction tube through the sleeve.

The present disclosure provides an anti-pollution consumable and a method for Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) molecular diagnosis using the same, belonging to the technical field of nucleic acid detection and molecular diagnostics. The anti-pollution consumable includes an outer reaction tube, a sleeve and an inner reaction tube, where the inner reaction tube includes a second hollow cylindrical upper body and a second-type conical lower body sequentially from top to bottom; a top end of the second hollow cylindrical upper body is externally connected with a fixing ring perpendicular to the second hollow cylindrical upper body; a number of drain holes are provided at a bottom of the second-type conical lower body; the drain hole has a diameter of 0.01-0.8 mm; the drain hole is used for hydrophobic treatment; and the inner reaction tube is fixed inside the outer reaction tube through the sleeve.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.