Single-cell analysis of a population of cells reveals cellular genotypes of individual cells. Accordingly, methods for performing single-cell analyses for a plurality of cells to determine cellular genotypes of individual cells are described. Generally, the single-cell Also described are methods of analysis involving targeted DNA-seq to generate sequence reads derived from genomic DNA that are used to determine the cell genotype. Methods described also include determining a cell genotype, particularly in distinguishing a genotype amongst a heterogenous population of cells, through analysis of different classes of cell mutations such as short-sequence mutations (e.g., SNVs) in combination with structural variants (e.g., CNVs). Reagents, materials, and kits for performing the same are also described. The identification of subpopulations of cells is informative for improving the understanding of cellular biology, especially in the context of diseases such as cancer, and is further informative for the better design of diagnostics and therapies.

Single-cell analysis of a population of cells reveals cellular genotypes of individual cells. Accordingly, methods for performing single-cell analyses for a plurality of cells to determine cellular genotypes of individual cells are described. Generally, the single-cell Also described are methods of analysis involving targeted DNA-seq to generate sequence reads derived from genomic DNA that are used to determine the cell genotype. Methods described also include determining a cell genotype, particularly in distinguishing a genotype amongst a heterogenous population of cells, through analysis of different classes of cell mutations such as short-sequence mutations (e.g., SNVs) in combination with structural variants (e.g., CNVs). Reagents, materials, and kits for performing the same are also described. The identification of subpopulations of cells is informative for improving the understanding of cellular biology, especially in the context of diseases such as cancer, and is further informative for the better design of diagnostics and therapies.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

Provided is a method capable of simply and exponentially amplifying circular DNA, and particularly, long-chain circular DNA, in a cell-free system. Specifically, provided herein is a method for amplifying circular DNA which comprises mixing circular DNA having a replication origin sequence (origin of chromosome (oriC)) with a reaction solution comprising: a first enzyme group that catalyzes replication of circular DNA; a second enzyme group that catalyzes an Okazaki fragment maturation and synthesizes two sister circular DNAs constituting a catenane; a third enzyme group that catalyzes a separation of two sister circular DNAs; and also, a buffer, NTP, dNTP, a magnesium ion source, and an alkali metal ion source, to form a reaction mixture, which is then reacted.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.