The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

The present disclosure provides compositions, methods and systems for quantifying target sequences and identifying target sequence variants.

The present disclosure provides compositions, methods and systems for quantifying target sequences and identifying target sequence variants.

Provided is a method capable of simply and exponentially amplifying circular DNA, and particularly, long-chain circular DNA, in a cell-free system. Specifically, provided herein is a method for amplifying circular DNA which comprises mixing circular DNA having a replication origin sequence (origin of chromosome (oriC)) with a reaction solution comprising: a first enzyme group that catalyzes replication of circular DNA; a second enzyme group that catalyzes an Okazaki fragment maturation and synthesizes two sister circular DNAs constituting a catenane; a third enzyme group that catalyzes a separation of two sister circular DNAs; and also, a buffer, NTP, dNTP, a magnesium ion source, and an alkali metal ion source, to form a reaction mixture, which is then reacted.

Provided herein are CD274 rearrangements. CD274 nucleic acid molecules, and PD-L1 polypeptides methods related to detecting CD274 rearrangements. CD274 nucleic acid molecules, and PD-L1 polypeptides in cancer, as well as methods of treatment and uses related thereto. Detection of CD274 rearrangements. CD274 nucleic acid molecules, and PD-L1 polypeptides of the disclosure can be used to identify individuals that may benefit from treatment with an anti-cancer therapy such as an immune checkpoint inhibitor.

Provided herein are CD274 rearrangements. CD274 nucleic acid molecules, and PD-L1 polypeptides methods related to detecting CD274 rearrangements. CD274 nucleic acid molecules, and PD-L1 polypeptides in cancer, as well as methods of treatment and uses related thereto. Detection of CD274 rearrangements. CD274 nucleic acid molecules, and PD-L1 polypeptides of the disclosure can be used to identify individuals that may benefit from treatment with an anti-cancer therapy such as an immune checkpoint inhibitor.

Provided is a method capable of simply and exponentially amplifying circular DNA, and particularly, long-chain circular DNA, in a cell-free system. Specifically, provided herein is a method for amplifying circular DNA which comprises mixing circular DNA having a replication origin sequence (origin of chromosome (oriC)) with a reaction solution comprising: a first enzyme group that catalyzes replication of circular DNA; a second enzyme group that catalyzes an Okazaki fragment maturation and synthesizes two sister circular DNAs constituting a catenane; a third enzyme group that catalyzes a separation of two sister circular DNAs; and also, a buffer, NTP, dNTP, a magnesium ion source, and an alkali metal ion source, to form a reaction mixture, which is then reacted.