A polynucleotide sequencing method includes a wash step that employs a composition including a polymerase. The composition may also include a plurality of nucleotides. The composition may be configured to prevent the polymerase from incorporating one of the plurality of nucleotides into a copy polynucleotide strand. The composition may be substantially free of Mg.sup.2+.

The present invention relates to: a composition for validating next generation sequencing (NGS) panels, comprising homozygote DNA and control genomic DNA; a kit for validation of NGS panels, comprising the composition; a validation method for NGS panels through the analysis of false negative variants, limit of detection, and false positive variants; and a method for providing information to enhance the specificity of NGS panels. In particular, the validation method of the present invention enables the objective analysis of the frequency of false negative variants, the frequency of false positive variants, and the limit of detection for NGS panels, making it effectively usable in the validation of NGS panels.

The present invention relates to: a composition for validating next generation sequencing (NGS) panels, comprising homozygote DNA and control genomic DNA; a kit for validation of NGS panels, comprising the composition; a validation method for NGS panels through the analysis of false negative variants, limit of detection, and false positive variants; and a method for providing information to enhance the specificity of NGS panels. In particular, the validation method of the present invention enables the objective analysis of the frequency of false negative variants, the frequency of false positive variants, and the limit of detection for NGS panels, making it effectively usable in the validation of NGS panels.