A digital droplet PCR method of analyzing the methylation status of methylation sites of a double-stranded DNA molecule which comprises at least two methylation sites per single strand of the double-stranded DNA molecule is disclosed.

A digital droplet PCR method of analyzing the methylation status of methylation sites of a double-stranded DNA molecule which comprises at least two methylation sites per single strand of the double-stranded DNA molecule is disclosed.

Methods and kits for determining the efficiency of plasma separation from whole blood are provided, using real-time PCR amplification of two amplicons, namely, a short amplicon of e.g. 70-150 bps and a long amplicon of e.g. 350-600 bps. The separation efficiency is determined based on the difference in amplification patterns of the two amplicons.

Methods and kits for determining the efficiency of plasma separation from whole blood are provided, using real-time PCR amplification of two amplicons, namely, a short amplicon of e.g. 70-150 bps and a long amplicon of e.g. 350-600 bps. The separation efficiency is determined based on the difference in amplification patterns of the two amplicons.

A digital droplet PCR method of analyzing the methylation status of methylation sites of a double-stranded DNA molecule which comprises at least two methylation sites per single strand of the double-stranded DNA molecule is disclosed.

A digital droplet PCR method of analyzing the methylation status of methylation sites of a double-stranded DNA molecule which comprises at least two methylation sites per single strand of the double-stranded DNA molecule is disclosed.

The present invention provides an oligonucleotide conjugate with high hybridization performance and use thereof. The oligonucleotide conjugate of the present invention can be used as a probe for nucleic acid detection, the oligonucleotide conjugate of the present invention can be used to design probes more flexibly, and the conservative regions are more accessible. The specificity of the probe is better, and the fluorescence value of the probe can be significantly improved, the fluorescence background is significantly reduced, and the sensitivity of the probe is greatly improved.

The present invention provides an oligonucleotide conjugate with high hybridization performance and use thereof. The oligonucleotide conjugate of the present invention can be used as a probe for nucleic acid detection, the oligonucleotide conjugate of the present invention can be used to design probes more flexibly, and the conservative regions are more accessible. The specificity of the probe is better, and the fluorescence value of the probe can be significantly improved, the fluorescence background is significantly reduced, and the sensitivity of the probe is greatly improved.

The carborhodamine dyes disclosed herein are novel reagents suitable for automated incorporation of carborhodamine dyes into oligonucleotides that can be used in detection methods for nucleic acid targets. This disclosure provides an efficient and simple process for the preparation of carborhodamine compounds and introduces previously unknown reagents for the automated synthesis of oligonucleotide-carborhodamine conjugates.

Provided herein are compositions, methods and uses that relate to an easy, accurate and reliable dual or duplex assay that determines both protein and nucleic acid amounts in a viral preparation in a single container and further determines full versus empty virion content.