The present invention provides a multiplex ligation-dependent probe microarray detection. Specifically, a surface probe-based quantitative PCR detection system in the present invention comprises: (a) a solid phase carrier; (b) a first probe; (c) a second probe; (d) a ligase for ligating the first probe and the second probe to form a third probe; (e) a quenching primer pair for amplifying the third probe; and (f) a quenching product capture nucleic acid immobilized on the solid phase carrier and matching a quenching amplification product. The present invention further provides a corresponding detection method and detection apparatus, and applications thereof. The present invention can rapidly, easily, efficiently, and simultaneously perform quantitative detection on multiple target sequences.

The present invention provides a multiplex ligation-dependent probe microarray detection. Specifically, a surface probe-based quantitative PCR detection system in the present invention comprises: (a) a solid phase carrier; (b) a first probe; (c) a second probe; (d) a ligase for ligating the first probe and the second probe to form a third probe; (e) a quenching primer pair for amplifying the third probe; and (f) a quenching product capture nucleic acid immobilized on the solid phase carrier and matching a quenching amplification product. The present invention further provides a corresponding detection method and detection apparatus, and applications thereof. The present invention can rapidly, easily, efficiently, and simultaneously perform quantitative detection on multiple target sequences.

Barcoded ligation assay products from individual samples.

Barcoded ligation assay products from individual samples.

A method of sequencing nucleic acids is provided using sequencing by ligation and/or sequencing by hybridization.

A method of sequencing nucleic acids is provided using sequencing by ligation and/or sequencing by hybridization.

This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.