A method for sequencing a nucleic acid strand, comprising the steps of: providing a solution containing truncated strands having lengths different from one another terminating with a respective dideoxynucleotide from among ddATP, ddTTP, ddGTP, and ddCTP; functionalizing first masses by a donor molecule and second masses by an acceptor molecule such as to generate a light emission when they come into mutual contact; coupling a first mass to a first end of each truncated strand; coupling the second masses to a respective terminal dideoxynucleotide of each strand; applying an AC electrical field having variable frequencies that are such as to generate, on each second mass, a net movement directed towards the first mass; acquiring a plurality of light radiations for each frequency value; and associating each light radiation acquired to a respective dideoxynucleotide and, thus, to a respective nucleotide base.

A method for sequencing a nucleic acid strand, comprising the steps of: providing a solution containing truncated strands having lengths different from one another terminating with a respective dideoxynucleotide from among ddATP, ddTTP, ddGTP, and ddCTP; functionalizing first masses by a donor molecule and second masses by an acceptor molecule such as to generate a light emission when they come into mutual contact; coupling a first mass to a first end of each truncated strand; coupling the second masses to a respective terminal dideoxynucleotide of each strand; applying an AC electrical field having variable frequencies that are such as to generate, on each second mass, a net movement directed towards the first mass; acquiring a plurality of light radiations for each frequency value; and associating each light radiation acquired to a respective dideoxynucleotide and, thus, to a respective nucleotide base.

The present disclosure provides an aqueous composition comprising a purified thermostable polypeptide with biological activity, and a purified thermolabile water-soluble substance which in a functional conformation at a permissive temperature absorbs light and/or exhibits fluorescence, wherein the maximal permissive temperature of the polypeptide is above the maximal permissive temperature of the substance. The present disclosure further provides methods employing such a composition, and kits containing ingredients to form such a composition.

The present disclosure provides an aqueous composition comprising a purified thermostable polypeptide with biological activity, and a purified thermolabile water-soluble substance which in a functional conformation at a permissive temperature absorbs light and/or exhibits fluorescence, wherein the maximal permissive temperature of the polypeptide is above the maximal permissive temperature of the substance. The present disclosure further provides methods employing such a composition, and kits containing ingredients to form such a composition.

The present invention concerns compositions and processes that use affinity tags for isolating, and detecting or quantifying analytes, including nucleic acids, proteins and polypeptides. Compositions include nucleic acid compositions and protein compositions with affinity binding pairs, including metal binding peptides and immobilized metals, or peptide affinity groups.

The present invention concerns compositions and processes that use affinity tags for isolating, and detecting or quantifying analytes, including nucleic acids, proteins and polypeptides. Compositions include nucleic acid compositions and protein compositions with affinity binding pairs, including metal binding peptides and immobilized metals, or peptide affinity groups.

The invention relates to a method for identifying and quantifying a polypeptide from a library of polypeptides. The method comprises the steps of: 1providing a polypeptide library and a detection tag library, 2generating a nested library comprising the polypeptides and the detection tags, 3sequencing the nested library, 4selecting a member of the nested library in one or several selection steps that are independent of a physical genotype-phenotype linkage, 5isolating the detection tag from the selected polypeptide, 6identifying and quantifying the detection tag by mass spectrometry, 7obtaining the sequence of the selected polypeptide. The invention also relates to a collection of polypeptides, a collection of detection tags, and a collection of plasmid vectors.

The invention relates to a method for identifying and quantifying a polypeptide from a library of polypeptides. The method comprises the steps of: 1providing a polypeptide library and a detection tag library, 2generating a nested library comprising the polypeptides and the detection tags, 3sequencing the nested library, 4selecting a member of the nested library in one or several selection steps that are independent of a physical genotype-phenotype linkage, 5isolating the detection tag from the selected polypeptide, 6identifying and quantifying the detection tag by mass spectrometry, 7obtaining the sequence of the selected polypeptide. The invention also relates to a collection of polypeptides, a collection of detection tags, and a collection of plasmid vectors.

Apparatus and means by which a polysaccharide or other heterogeneous polymer is concatenated with a nucleic acid polymer that is captured by a primer on a polymerase tethered to a bead trapped by a nanopore. The translocation of the nanopore by the polysaccharide or other heterogeneous polymer is then controlled by the speed at which the polymerase releases newly synthesized nucleic acid or slows the motion of the nucleic acid as it is pulled on by an electrophoretic field.

Apparatus and means by which a polysaccharide or other heterogeneous polymer is concatenated with a nucleic acid polymer that is captured by a primer on a polymerase tethered to a bead trapped by a nanopore. The translocation of the nanopore by the polysaccharide or other heterogeneous polymer is then controlled by the speed at which the polymerase releases newly synthesized nucleic acid or slows the motion of the nucleic acid as it is pulled on by an electrophoretic field.