The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.

The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

Provided herein are methods for enhanced specificity of multiplexed measurements. Methods provided herein include immunoassay reactions and/or measuring protein-protein interactions with direct sequencing readouts of DNA barcodes.

Provided herein are methods for enhanced specificity of multiplexed measurements. Methods provided herein include immunoassay reactions and/or measuring protein-protein interactions with direct sequencing readouts of DNA barcodes.

The present invention is directed to a method for extraction of cell-free nucleic acid fragments from a blood sample to facilitate cancer diagnosis, prognosis and monitoring as well as prenatal screening. The present invention provides a cartridge comprising a first compartment for filtering plasma from a blood sample and preferably also for cell fixation and cell rinsing in order to improve yield and a second compartment for performing nucleic acid separation, wherein the first compartment comprises a hollow fiber membrane and the second compartment comprises material for binding the nucleic acids or a gel for electrophoresis. The invention also provides and an automated system comprising a device with a docking site adapted to receive said cartridge, said device comprising means adapted to operate the blood plasma filtering process in said cartridge and means adapted to operate nucleic acid separation in said cartridge.

The present invention is directed to a method for extraction of cell-free nucleic acid fragments from a blood sample to facilitate cancer diagnosis, prognosis and monitoring as well as prenatal screening. The present invention provides a cartridge comprising a first compartment for filtering plasma from a blood sample and preferably also for cell fixation and cell rinsing in order to improve yield and a second compartment for performing nucleic acid separation, wherein the first compartment comprises a hollow fiber membrane and the second compartment comprises material for binding the nucleic acids or a gel for electrophoresis. The invention also provides and an automated system comprising a device with a docking site adapted to receive said cartridge, said device comprising means adapted to operate the blood plasma filtering process in said cartridge and means adapted to operate nucleic acid separation in said cartridge.

Provided herein are systems and methods for detecting genomic structural variants using a non-application gene-editing sample preparation followed by long-read sequencing.

Provided herein are systems and methods for detecting genomic structural variants using a non-application gene-editing sample preparation followed by long-read sequencing.