The present disclosure provides, in some aspects, methods and compositions for producing nucleic acid nanostructures having little to no kinetic barriers to self-assembly.

The present disclosure provides, in some aspects, methods and compositions for producing nucleic acid nanostructures having little to no kinetic barriers to self-assembly.

A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.

A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.

An automated multiplex detector system includes: (a) a nucleic acid amplification compartment for amplifying nucleic acid of one or more targets in a sample, and (b) an analysis compartment in fluid communication with the amplification compartment, the analysis compartment housing a nanoparticle-based multiplex detector capable of using the amplified nucleic acid of the amplification compartment and producing a signal that correlates with the presence of the one or more targets in the sample.

An automated multiplex detector system includes: (a) a nucleic acid amplification compartment for amplifying nucleic acid of one or more targets in a sample, and (b) an analysis compartment in fluid communication with the amplification compartment, the analysis compartment housing a nanoparticle-based multiplex detector capable of using the amplified nucleic acid of the amplification compartment and producing a signal that correlates with the presence of the one or more targets in the sample.

Plasmonics-active nanoprobes are provided for detection of target biomolecules including nucleic acids, proteins, and small molecules. The nucleic acids that can be detected include RNA, DNA, mRNA, microRNA, and small nucleotide polymorphisms (SNPs). The nanoproprobes can be used in vito in sensitive detection methods for diagnosis of diseases and disorders including cancer. Multiplexing can be performed using the nanoprobes such that multiple targets can be detected simultaneously in a single sample. The methods of use of the nanoprobes include detection by a visible color change. The nanoprobes can be used in vivo for treatment of undesireable cells in a subject.

Plasmonics-active nanoprobes are provided for detection of target biomolecules including nucleic acids, proteins, and small molecules. The nucleic acids that can be detected include RNA, DNA, mRNA, microRNA, and small nucleotide polymorphisms (SNPs). The nanoproprobes can be used in vito in sensitive detection methods for diagnosis of diseases and disorders including cancer. Multiplexing can be performed using the nanoprobes such that multiple targets can be detected simultaneously in a single sample. The methods of use of the nanoprobes include detection by a visible color change. The nanoprobes can be used in vivo for treatment of undesireable cells in a subject.

The disclosure relates to the use of nanoparticles that are coated with unique oligonucleotide (e.g., DNA) sequences of various base lengths (“nano-DNA”) that act as barcodes for product authentication, product serialization, brand protection, track-and-trace, intelligent supply chain, and law enforcement. The nano-DNA can be incorporated into inks, dyes, resins, labels, and other markings at all manufacturing levels, including the product (unit) level, to encode company and product-specific information. The nano-DNA can also be embedded in the product itself during the manufacturing process. Furthermore, the nano-DNA can be quickly, simply, and inexpensively monitored and verified using an electrochemical biosensor device in resource-limited field conditions.

The disclosure relates to the use of nanoparticles that are coated with unique oligonucleotide (e.g., DNA) sequences of various base lengths (“nano-DNA”) that act as barcodes for product authentication, product serialization, brand protection, track-and-trace, intelligent supply chain, and law enforcement. The nano-DNA can be incorporated into inks, dyes, resins, labels, and other markings at all manufacturing levels, including the product (unit) level, to encode company and product-specific information. The nano-DNA can also be embedded in the product itself during the manufacturing process. Furthermore, the nano-DNA can be quickly, simply, and inexpensively monitored and verified using an electrochemical biosensor device in resource-limited field conditions.