A digital assay for a micro RNA (miRNA) or other target analyte in a sample makes use of nanoparticles that absorb light at the resonant wavelength of a photonic crystal (PC). Such nanoparticles locally quench the resonant reflection of light from the PC when present on the surface of the PC. The nanoparticles are functionalized to specifically bind to the target analyte, and the PC surface is functionalized to specifically bind to the nanoparticles that have bound to the target analyte. The sample is exposed to the functionalized nanoparticles, and the individual nanoparticles bound to the PC surface can be identified and counted based on reduced intensity values in the reflected light from the PC. The number of bound nanoparticles that are counted in this way can be correlated to the abundance of the target analyte in the sample.

Provided herein is a method for isolating high molecular weight (HMW) DNA using beads that are at least 200 μm in diameter that utilizes a device for retaining the beads and where the purified DNA eluant exits the device without shearing the HMW DNA. In some embodiments, the method comprises precipitating the DNA onto the beads, washing the beads in the device, and then eluting the DNA from the beads therein while substantially avoiding shear. Compositions and kits for practicing the method are also provided.

Provided herein is a method for isolating high molecular weight (HMW) DNA using beads that are at least 200 μm in diameter that utilizes a device for retaining the beads and where the purified DNA eluant exits the device without shearing the HMW DNA. In some embodiments, the method comprises precipitating the DNA onto the beads, washing the beads in the device, and then eluting the DNA from the beads therein while substantially avoiding shear. Compositions and kits for practicing the method are also provided.

The present invention relates to: a single nanoparticle biosensor platform comprising a metal nanoparticle in which a biomolecule is immobilized between two metal nanoseeds, and a biosensor comprising same; a method for detecting mutations by using the biosensor; and a method for manufacturing a single nanoparticle biosensor platform, comprising a step of forming a metal nanoparticle in which a biomolecule is immobilized between two metal nanoseeds. The single nanoparticle biosensor platform according to the present invention enables high sensitivity and reliability detection of a target, and also enables direct identification of various mutations so as to enable the efficient diagnosis of mutations, thereby being widely usable in the biomedical diagnostic fields and the like.

The present invention relates to: a single nanoparticle biosensor platform comprising a metal nanoparticle in which a biomolecule is immobilized between two metal nanoseeds, and a biosensor comprising same; a method for detecting mutations by using the biosensor; and a method for manufacturing a single nanoparticle biosensor platform, comprising a step of forming a metal nanoparticle in which a biomolecule is immobilized between two metal nanoseeds. The single nanoparticle biosensor platform according to the present invention enables high sensitivity and reliability detection of a target, and also enables direct identification of various mutations so as to enable the efficient diagnosis of mutations, thereby being widely usable in the biomedical diagnostic fields and the like.

Disclosed are compositions and methods for the multiplexed analysis of one or more intracellular targets of a single cell. Exemplary compositions of the disclosure comprise a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and wherein the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and wherein each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; a coating composition comprising a cell lysis composition; and a linker composition comprising a functionalization component and an extension component.

Disclosed are compositions and methods for the multiplexed analysis of one or more intracellular targets of a single cell. Exemplary compositions of the disclosure comprise a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and wherein the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and wherein each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; a coating composition comprising a cell lysis composition; and a linker composition comprising a functionalization component and an extension component.

The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.

The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.

The present disclosure is directed to spherical nucleic acids (SNAs) comprising a nanoparticle core and an oligonucleotide, use of the SNAs to, e.g., detect target analytes, and methods of making the SNAs. In various embodiments, the target analyte is detected using the nanoparticle core, the oligonucleotide, or both. In some embodiments, the oligonucleotide comprises a detectable marker situated at an internal location within the oligonucleotide. In some aspects, the disclosure provides methods for detecting a target analyte comprising the step of contacting the target analyte with a spherical nucleic acid (SNA) and an agent, the SNA comprising a protein core and an oligonucleotide attached thereto, wherein the contacting of the protein core with the target analyte results in a change in the target analyte that is detectable by the agent, thereby detecting the target analyte.